/qresearch/ - Q Research★

DARPA, Bill Gates, Pfizer, BioNTech, Rockefeller, WHO, CDC, Moderna Tx, Profusa, John Hopkins, Harvard, MIT and UN

Event 201: Simulation of Coronavirus Outbreak

https://www.centerforhealthsecurity.org/event201/about

It wouldn't by any chance be the same digital currency that stores Human DNA? https://businessblockchainhq.com/business-blockchain-news/blockchain-to-store-human-dna/

Does it have the same affiliation with Accenture & Hyperledger?

Accenture

https://www.accenture.com/us-en/insight-blockchain-id2020

Hyperledger

https://www.hyperledger.org/news/2018/01/23/1-23-18-coindesk-microsoft-hyperledger-un-join-blockchain-identity-initiative

DARPA:

Bill Gates Partners DARPA For New DNA Nanotech COVID-19 Vaccine

https://www.mirrorreview.com/bill-gates-partners-darpa-for-covid-19-vaccine/

DARPA - Connection to IBM

https://www-03.ibm.com/press/us/en/pressrelease/20671.wss

DARPA - Connection to CureVac

https://globalbiodefense.com/newswire/curevac-ceo-white-house-coronavirus-task-force-meeting-mrna-vaccines/

DARPA - Connection to Moderna

https://investors.modernatx.com/news-releases/news-release-details/darpa-awards-moderna-therapeutics-grant-25-million-develop

DARPA - Connection to Pfizer

https://www.fool.com/investing/general/2013/12/05/darpa-hires-pfizer-to-perform-groundbreaking-vacci.aspx

DARPA Connection with Sigma Aldrich (Lucierfense Enzyme)

DARPA - Connection to Sigma Aldrich - DARPA Antibody Technology Program, Phase II: Characterization of an Anti-BclA Antibody Produced by U.S. Naval Research Laboratory

https://apps.dtic.mil/sti/pdfs/AD1074346.pdf

DARPA - Selection of cost-effective yet chemically diverse pathways from the networks of computer-generated retrosynthetic plans

https://pubs.rsc.org/en/content/articlehtml/2019/sc/c8sc05611k

Sigma Aldrich Meet Synthia™ - The Future of Retro Software you easily analyze custom pathways for known and novel molecules against your search criteria

https://www.sigmaaldrich.com/chemistry/chemical-synthesis/synthesis-software.html

DARPA Tests Advanced Chemical Sensors

https://www.darpa.mil/news-events/2019-04-30

Video ChemSIGMA DARPA Tests Advanced Chemical Sensors

https://www.youtube.com/watch?v=95vqUNilZKc&feature=emb_title

Chematica: A Story of Computer CodeThat Started to Think like a Chemist

https://www.cell.com/chem/pdf/S2451-9294(18)30085-8.pdf

Bill Gates

Bill Gates Partners DARPA For New DNA Nanotech COVID-19 Vaccine

https://www.mirrorreview.com/bill-gates-partners-darpa-for-covid-19-vaccine/

Bill Gates

Scientists at The Pirbright Institute will receive a subaward of $2.66 million as part of a research team led by Massachusetts Institute of Technology (MIT) in DARPA's Safe Genes programme.

https://www.pirbright.ac.uk/news/2017/12/research-funding-safe-genes

DARPA - Bill & Melinda Gates Foundation

Dr. Chitale has consulted with organizations including the Bill & Melinda Gates Foundation, PATH, the World Health Organization (WHO), Wyeth Pharmaceuticals, and Thomson Reuters, Inc.

https://www.darpa.mil/staff/dr-rohit-chitale

Bill Gates & Rockefeller

Are Gates and Rockefeller using their influence to set agenda in poor states?

https://www.theguardian.com/global-development/2016/jan/15/bill-gates-rockefeller-influence-agenda-poor-nations-big-pharma-gm-hunger

Bill Gates & GlaxoSmithKline (GSK)

Bill Gates connected Big Pharma Exec to head vaccine developments (GlaxoSmithKline)

https://nexusnewsfeed.com/article/human-rights/bill-gates-connected-big-pharma-exec-to-head-vaccine-developments

Bill Gates & Epstein

Bill Gates made donations to MIT through Jeffrey Epstein ---here are all of the tech mogul's connections to the financier.

https://www.businessinsider.com/bill-gates-connections-jeffrey-epstein-mit-donations-ronan-farrow-2019-9?op=1

Bill Gates Worked With Epstein On A Pedophile Project Involving Children In Developing Countries

https://fort-russ.com/2020/04/bill-gates-worked-with-epstein-on-a-pedophile-project-involving-children-in-developing-countries/?fbclid=IwAR19_yhTuh5ibG0b0ZlsWAjYpZ4t51k_IUnlnEW6Og7pC2DzuU2ddAhzyT4

Bill Gates & Gavi

Gates Foundation pledged US$ 750 million to set up Gavi in 1999. The Foundation is a key Gavi partner in vaccine market shaping.

https://www.gavi.org/operating-model/gavis-partnership-model/bill-melinda-gates-foundation

Rockefeller Foundation

Gavi receives US$ 5 million from The Rockefeller Foundation

June 1st 2020

https://www.gavi.org/news/media-room/gavi-receives-us-5-million-rockefeller-foundation

Rockefeller Foundation Launches $100 Million Public Health Initiative - September 2019

https://philanthropynewsdigest.org/news/rockefeller-foundation-launches-100-million-public-health-initiative

Rockefeller and Gates Foundation Team Up

http://archive.wphna.org/wp-content/uploads/2015/04/2014-11-Hypothesis-Anne-Emanuelle-Birn-Rockefeller-and-Gates.pdf

Are Gates and Rockefeller using their influence to set agenda in poor states?

January 15th 2016

https://www.theguardian.com/global-development/2016/jan/15/bill-gates-rockefeller-influence-agenda-poor-nations-big-pharma-gm-hunger

Rockefeller Lockstep

http://www.nommeraadio.ee/meedia/pdf/RRS/Rockefeller%20Foundation.pdf

Pfizer and BioNTech Announce an Agreement with U.S. Government for up to 600 Million Doses of mRNA-based Vaccine Candidate Against SARS-CoV-2

https://www.pfizer.com/news/press-release/press-release-detail/pfizer-and-biontech-announce-agreement-us-government-600

Pfizer Awarded DARPA Biodefense Contract -- DoD Daily Contracts

https://news.clearancejobs.com/2013/12/04/pfizer-awarded-darpa-biodefense-contract-dod-daily-contracts/

DARPA Hires Pfizer to Perform Groundbreaking Vaccine Research

https://www.fool.com/investing/general/2013/12/05/darpa-hires-pfizer-to-perform-groundbreaking-vacci.aspx

Pfizer agrees to manufacture Gilead’s coronavirus drug remdesivir

https://www.cnbc.com/2020/08/07/pfizer-agrees-to-manufacture-gileads-coronavirus-drug-remdesivir.html

Pfizer Collaborates with Gene Editing Company Sangamo Therapeutics on Hemophilia A Treatment

https://www.pharmasalmanac.com/articles/pfizer-collaborates-with-gene-editing-company-sangamo-therapeutics-on-hemophilia-a-treatment

Pfizer Partners with Gavi

https://www.pfizer.com/science/vaccines/global-impact/our-impact-on-innovation

Pfizer Partners With Gavi to Supply Our Vaccine to 50 Countries

https://www.pfizer.com/sites/default/files/investors/financial_reports/annual_reports/2018/our-culture-our-purpose/our-purpose/supply-prevenar/index.html

Bio Roundup: Pfizer’s Future, CRISPR in Patients, Drug Imports & More

https://xconomy.com/national/2019/08/02/bio-roundup-pfizers-future-crispr-in-patients-drug-imports-more/

Pfizer, BioNTech Begin Phase II/III Trial of COVID-19 Vaccine, Switching Lead Candidate

https://www.genengnews.com/news/pfizer-biontech-begin-phase-ii-iii-trial-of-covid-19-vaccine-switching-lead-candidate/

BioNTech

BioNTech partners with Bill and Melinda Gates Foundation, scoring $55M equity investment; BeiGene brushes off short attack

https://endpts.com/biontech-partners-with-bill-and-melinda-gates-foundation-scoring-55m-equity-investment-novartis-sells-china-unit/

Bill & Melinda Gates Foundation Strategic Investment Fund

https://sif.gatesfoundation.org/investments/biontech/

EU In Talks With Moderna, BioNtech, CureVac For Potential Covid-19 Vaccine Deals

https://financemarkethouse.com/2020/07/19/eu-in-talks-with-moderna-biontech-curevac-for-potential-covid-19-vaccine-deals/

BioNTech and Genevant Sciences Sign Strategic mRNA-Focused Partnership in Rare Diseases

https://genevant.com/biontech-and-genevant-sciences-sign-strategic-mrna-focused-partnership-in-rare-diseases/

BioNTech’s COVID-19 Plan

https://biontech.de/covid-19

BioNTech, Genevant form mRNA pacts in rare diseases, oncology

https://www.fiercebiotech.com/biotech/biontech-genevant-form-mrna-pacts-rare-diseases-oncology

Pfizer, BioNTech Begin Phase II/III Trial of COVID-19 Vaccine, Switching Lead Candidate

https://www.genengnews.com/news/pfizer-biontech-begin-phase-ii-iii-trial-of-covid-19-vaccine-switching-lead-candidate/

Pfizer and BioNTech Announce an Agreement with U.S. Government for up to 600 Million Doses of mRNA-based Vaccine Candidate Against SARS-CoV-2

https://www.pfizer.com/news/press-release/press-release-detail/pfizer-and-biontech-announce-agreement-us-government-600

U.S. agrees to pay Pfizer and BioNTech $2 billion for 100 million doses of coronavirus vaccine

https://www.cnbc.com/amp/2020/07/22/us-government-taps-pfizer-to-produce-millions-of-doses-of-coronavirus-vaccine.html

Gilead Science - Cell Signaling - Luciferase - Essential For Remote Control CRISPR

Gilead Sciences CLINICAL STUDY PROTOCOL

https://clinicaltrials.gov/ProvidedDocs/60/NCT02943460/Prot_001.pdf

A nanoluciferase SARS-CoV-2 for rapid neutralization testing and screening of anti-infective drugs for COVID-19

https://www.biorxiv.org/content/10.1101/2020.06.22.165712v1

Covid-19 Small Molecule Therapies Reviewed - Gilead Science

https://blogs.sciencemag.org/pipeline/archives/2020/03/06/covid-19-small-molecule-therapies-reviewed

Discovery and Synthesis of a Phosphoramidate Prodrug of a Pyrrolo[2,1-f][triazin-4-amino] Adenine C-Nucleoside (GS-5734) for the Treatment of Ebola and Emerging Viruses

https://pubs.acs.org/doi/10.1021/acs.jmedchem.6b01594

Sensitive bioluminescence imaging of fungal dissemination tothe brain in mouse models of cryptococcosis

Pathogen of interest to express a luciferase gene allows for real-time. K.L. has received research grants and travel support from Gilead, MSD and Pfizer

https://dmm.biologists.org/content/dmm/12/6/dmm039123.full.pdf

MicroRNA let-7-TGFBR3 signalling regulates cardiomyocyte apoptosis after infarction - research support from AstraZeneca, Takeda, and Gilead

https://www.sciencedirect.com/science/article/pii/S2352396419305171

Gilead and Sangamo deal shows that CRISPR still has competition (Zinc Finger Nuclease Gene Editing alternative to CRISPR)

https://cen.acs.org/articles/96/web/2018/02/Gilead-Sangamo-deal-shows-CRISPR.html

How Gilead Sciences Is Betting Big on Gene Editing

https://www.fool.com/investing/2018/02/23/how-gilead-sciences-is-betting-big-on-gene-editing.aspx

CRISPR-engineered T cells in patients with refractory cancer - Nalm-6 tumor cells engineered to express NY-ESO-1 and luciferase

http://pibmub.com/eaba7365.html

Luciferase gene-loaded CS-Qdots as self-illuminating probes for specific hepatoma imaging

https://pubs.rsc.org/--/content/articlelanding/2015/ra/c5ra01508a#!divAbstract

Luciferase gene-loaded CS-Qdots

https://pubs.rsc.org/- -/content/articlelanding/2015/ra/c5ra01508a#!divAbstract

BlackRock

Leading Pfizer and AstraZeneca shareholder BlackRock wants the megamerger

https://www.fiercepharma.com/m-a/leading-pfizer-and-astrazeneca-shareholder-blackrock-wants-megamerger

BlackRock Inc. reports 60.22% increase in ownership of MRNA / Moderna Inc

https://fintel.io/so/us/mrna/blackrock

BlackRock Inc. discloses 7.50% ownership in GSK / GlaxoSmithKline plc

https://fintel.io/so/us/gsk/blackrock

CureVac Connections Both Bill Gates & DARPA

CureVac - Bill & Melinda Gates Foundation

These Are The Billionaire Investors Behind German Drugmakers Developing A Coronavirus Vaccine

https://www.forbes.com/sites/martaorosz/2020/03/16/these-are-the-billionaire-investors-behind-german-drugmakers-developing-a-coronavirus-vaccine/#1198be801fb8

CureVac - DARPA

CureVac, Sanofi Pasteur, and In-Cell-Art Collaborate on €33.1M DARPA-Supported Vaccine Program

https://www.genengnews.com/topics/drug-discovery/curevac-sanofi-pasteur-and-in-cell-art-collaborate-on-e33-1m-darpa-supported-vaccine-program/

Castlerock Asset Management Inc

Castle Rock Wealth Management, Llc ownership in GILD / Gilead Sciences, Inc.

https://fintel.io/so/us/gild/castle-rock-wealth-management

Castle Rock Wealth Management, Llc reports 72.34% increase in ownership of MMM / 3M Co

https://fintel.io/so/us/mmm/castle-rock-wealth-management

Castle Rock Wealth Management, Llc reports 40.83% increase in ownership of MSFT / Microsoft Corp

https://fintel.io/so/us/msft/castle-rock-wealth-management

Castle Rock Wealth Management, Llc reports 64.82% increase in ownership of QCOM / QUALCOMM Incorporated

https://fintel.io/so/us/qcom/castle-rock-wealth-management

Castlerock Asset Management Inc - Google, Ebay, Qualcomm

https://fintel.io/i13f/castlerock-asset-management/2014-09-30-0

Castlerock Asset Management Inc - Composition of Holdings by Industry

https://fintel.io/iind/castlerock-asset-management

Castle Rock Wealth Management, Llc reports 0.28% decrease in ownership of BLK / BlackRock, Inc.

https://fintel.io/so/us/blk/castle-rock-wealth-management

Castle Rock Wealth Management, Llc - Latest 13F Holdings, Performance, AUM

https://fintel.io/i/castle-rock-wealth-management

Rockefeller Connections

The Covid-Plan / Rockefeller Lockstep 2010

https://stateofthenation.co/?p=20234

Key Findings and Lessons from an Evaluation of the Rockefeller Foundation's Disease Surveillance Networks Initiative

https://web.archive.org/web/20200616010829/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3557955/

Are Gates and Rockefeller using their influence to set agenda in poor states?

January 15th 2016

https://www.theguardian.com/global-development/2016/jan/15/bill-gates-rockefeller-influence-agenda-poor-nations-big-pharma-gm-hunger

Backstage: the relationship between the Rockefeller Foundation and the World Health Organization, Part I: 1940s-1960s

https://pubmed.ncbi.nlm.nih.gov/24412372/

U.S. Philanthrocapitalism and the Global Health Agenda: The Rockefeller and Gates Foundations, Past and Present

May 16th 2016

https://www.globalpolicy.org/home/270-general/52947-us-philanthrocapitalism-and-the-global-health-agenda-the-rockefeller-and-gates-foundations-past-and-present.html

World Bank Group:

World Bank Document Lists COVID-19 Program Ending in March 2025

https://nationalfile.com/world-bank-document-lists-covid-19-program-ending-in-march-2025/

DARPA

*SAFE GENES PROGRAM:

The Safe Genes program supports force protection and military health and readiness by protecting Service members from accidental or intentional misuse of genome editing technologies. Additional work will leverage advances in gene editing technology to expedite development of advanced prophylactic and therapeutic treatments against gene editors. Advances within the program will ensure the United States remains at the vanguard of the broadly accessible and rapidly progressing field of genome editing.

https://www.darpa.mil/program/safe-genes

*DARPA SEEKS PATHOGEN DETECTORS THAT USE CRISPR:

DARPA Seeks Pathogen Detectors That Use CRISPR to Run 1,000 Tests at Once - 2019:

https://spectrum.ieee.org/the-human-os/biomedical/diagnostics/darpa-wants-scifi-pathogen-detectors

*DARPA FUNDS RESEARCH ON CONTROLLING, INHIBITING & REVERSING GENE EDITING:

DARPA to Fund Research on Controlling, Inhibiting, and Reversing Genome Editing:

https://scipol.org/news/darpa-fund-research-controlling-inhibiting-and-reversing-genome-editing

LUCIFERASE ROLE WITH CRISPR

Rapid Generation of Endogenously Driven Transcriptional Reporters in Cells Through CRISPR-CAS9

In order to generate a sensitive transcriptional reporter capable of being exploited for high throughput screens, we used a firefly luciferase cassette downstream of the endogenous promoter of the PAI-1 gene. We took advantage of the non-tagged GFP downstream of luciferase to ensure fluorescence-based detection of the cassette delivery and isolation of luciferase positive clones. Using recently developed super-efficient CRISP/Cas9 system in a doxycycline-inducible osteosarcoma U2OS Stably Expressing Cas9-Cells (SEC-C),32 we co-transfected a vector encoding sgRNA targeting the start codon region of the PAI-1 gene (sgPAI-1) together with the TGFβ reporter donor vector containing two homologous regions (~500nts) with the PAI-1 gene, one upstream (L-Arm) and one downstream (R-Arm) of the luciferase and GFP reporter cassette, to allow for homologous recombination (Fig. 1a). To ensure the integrity of the R-Arm, we introduced silent mutations on the sgPAI-1 recognition site. The reporter cassette in the donor vector encodes the firefly luciferase enzyme cDNA, which following homologous recombination overlaps perfectly with the start codon (ATG) of the endogenous PAI-1 gene (Fig. 1a). Directly downstream of the luciferase gene, we incorporated an IRES-GFP-2a cassette. The IRES element ensures the expression of GFP separately from the luciferase protein, while the 2a self-cleaving peptide ensures the cleavage of the GFP protein from the endogenous PAI-1 further downstream, both in frame. We call this unique targeting strategy: the second-generation reporter (2G-reporter) system.

https://www.nature.com/articles/srep09811

Novel CRISPR Applications

Endogenous Luciferase Reporter:

Common protocol to measure the affect of extracellular signals on protein expression is to transfect a cell line with a plasmid expressing a reporter (such as luciferase) under the control of known transcriptional control elements.

These plasmids were co-transfected into a human leukemia cell line in order to produce a double stranded break to knockout 1 allele of the target gene while simultaneously knocking-in the luciferase gene. The final cell line contained one natural allele and one luciferase allele, both controlled under the same endogenous enhancer/promoter region.

https://www.genscript.com/crispr-blog-what-is-crispr.html

Luciferase assays for C. parvum drug resistance and CRISPR/Cas9 activity.

https://www.researchgate.net/figure/Luciferase-assays-for-C-parvum-drug-resistance-and-CRISPR-Cas9-activity-a-HCT-8-cells_fig3_280121773

Luciferase Assays and Reporters dual-reporter luminescence system, which allows reporter assays in live cells.

https://www.genecopoeia.com/product/luciferase-assays/

The luciferase reporter system of the MMP12 endogenous promoter for investigating transcriptional regulation of the human MMP12 gene

https://www.sciencedirect.com/science/article/pii/S0717345819300521

*LUCIFERASE GENE INSERT

How to insert a reporter gene using CRISPR/Cas9 method?

https://www.researchgate.net/post/How_to_insert_a_reporter_gene_using_CRISPR_Cas9_method

DNA scaffold nanostructures for efficient and directional propagation of light harvesting cascades

https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10355/1035503/DNA-scaffold-nanostructures-for-efficient-and-directional-propagation-of-light/10.1117/12.2274639.short

Site-Specific Gene Knock-Out and On-Site Heterologous - Frontiers

https://www.frontiersin.org/articles/10.3389/fpls.2020.00306/full

**CRISPR-CAS9 KNOCK IN Cell TAGGING

CRISPR Cas9 Knock In Tagging

https://www.promega.com/products/small-molecule-drug-discovery/crispr-cas9-cell-lines-knock-in-tagging/

**CRISPR-CAS9 KNOCK IN USING STEM CELLS

One-Step Generation of Seamless Luciferase Gene Knockin Using CRISPR/Cas9 Genome Editing in Human Pluripotent Stem Cells

https://experiments.springernature.com/articles/10.1007/978-1-4939-9080-1_5

*LUCIFERASE LIGHTSWITCH ASSAY SYSTEM - ON / OFF SYSTEM:

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

https://www.jove.com/t/3343/identifying-targets-human-micrornas-with-lightswitch-luciferase-assay

That is essential for creating Bioluminescence in your cells. It will make it easier for 24 hour monitoring. Also they will be sending specific information using light - to your body that will activate CRISPR allowing for this.

5 WAYS TO GET CRISPR INSIDE OF YOU!

1) Gels & Creams

2) Editable & Drinkable

3) Ear Injections

4) Skin Graft

5) Ex-Vivo Therapy

https://www.technologyreview.com/2017/09/22/149011/five-ways-to-get-crispr-into-the-body/

USING LIGHT CONTROL CRISPR

Scientists Control CRISPR With Light

https://spectrum.ieee.org/the-human-os/biomedical/devices/light-activated-gene-editing

Using Light to Control Genome Editing

https://neurosciencenews.com/light-crispr-cas9-4917/

MIT - Remote Control of Gene Editing with Light

https://www.chemistryviews.org/details/ezine/9926991/Remote_Control_of_Gene_Editing_with_Light.html

Engineering a far-red light--activated split-Cas9 system for remote-controlled genome editing of internal organs and tumors

https://advances.sciencemag.org/content/6/28/eabb1777

*REMOTE CONTROLLED GENE EDITING PLATFORM:

Near-infrared upconversion--activated CRISPR-Cas9 system: A remote-controlled gene editing platform

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447385/

*LIGHT ACTIVATED CRISPR EDITING USING GUIDED RNA:

Development of light-activated CRISPR using photocleavable protectors of guide RNAs

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5864249/

Light-activated 'CRISPR' enables fast, precise gene editing and detection of DNA repair

https://www.nsf.gov/discoveries/disc_summ.jsp?cntn_id=300807&org=NSF&from=news

*IN VIVO CRISPR-CAS9 GENE EDITING WITH CONTROLLABLE LOCATION & TIME:

Light-responsive charge-reversal nanovector for high-efficiency in vivo CRISPR/Cas9 gene editing with controllable location and time

https://link.springer.com/article/10.1007/s12274-020-2864-z

*ON / OFF SWITCH FOR GENES:

Photoswitchable gRNAs for Spatiotemporally Controlled CRISPR-Cas-Based Genomic Regulation - On / Off Switch for Genes

https://pubs.acs.org/doi/10.1021/acscentsci.9b01093

PDF Link: https://pubs.acs.org/doi/pdf/10.1021/acscentsci.9b01093

REMOTE CONTROLLED GENOME EDITING

Remote-Controlled Genome Editing Of Internal Organs And Tumors

https://www.pioneeringminds.com/remote-controlled-genome-editing-internal-organs-tumors/

TRANSFECTION METHODS FOR CRISPR-CAS9

A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing

https://www.thno.org/v10p5532.htm

JOHN HOPKINS CONTRIBUTION FOR THE NWO

John Hopkins Attended Event 201. There contribution in this Plandemic was creating 2 very specific trigger methods for CRISPR-CAS9.

*TWO UNIQUE TRIGGER METHODS FOR CRISPR

FIRST METHOD (MASSIVELY IMPORTANT) - RNA VACCINE DEPENDANT ACTIVATION

Once RNA vaccine enters your body Luciferase Gene gets inserted into your DNA causing a chain reaction that now modifies your body allowing all new cells that are created to be created with the Luciferase Enzyme already inside each new cell this will allow for bioluminescence.

John Hopkins - 1st Method: Speedy Cuts: CRISPR On-Demand Method Developed (Once RNA Vaccine Enters Your Body)

https://www.genengnews.com/news/speedy-cuts-crispr-on-demand-method-developed/

SECOND METHOD - DEPENDANT UPON THE FIRST METHOD (LUCIFERASE GENE INSERTION)

How the New World Order maintains control of people is by using light activated CRISPR-CAS9 for remote control gene editing. The 2nd Trigger Method is for Remote Control Gene Editing. This method uses light to send a guided RNA method to CRISPR-CAS9 allowing the controller to grow Tumors, Cancer or Trigger Organ Failure.

John Hopkins - 2nd Method: Light-Activated 'CRISPR' Triggers Precision Gene Editing and Super-Fast DNA Repair

https://www.hopkinsmedicine.org/news/newsroom/news-releases/light-activated-crispr-triggers-precision-gene-editing-and-super-fast-dna-repair

JOHN HOPKINS CONTRIBUTION FOR THE NWO

John Hopkins Attended Event 201. There contribution in this Plandemic was creating 2 very specific trigger methods for CRISPR-CAS9.

TWO UNIQUE TRIGGER METHODS FOR CRISPR

*FIRST METHOD (MASSIVELY IMPORTANT) - RNA VACCINE DEPENDANT ACTIVATION*

Once RNA vaccine enters your body Luciferase Gene gets inserted into your DNA causing a chain reaction that now modifies your body allowing all new cells that are created to be created with the Luciferase Enzyme already inside each new cell this will allow for bioluminescence.

John Hopkins - 1st Method: Speedy Cuts: CRISPR On-Demand Method Developed (Once RNA Vaccine Enters Your Body)

https://www.genengnews.com/news/speedy-cuts-crispr-on-demand-method-developed/

*SECOND METHOD - DEPENDANT UPON THE FIRST METHOD (LUCIFERASE GENE INSERTION)*

How the New World Order maintains control of people is by using light activated CRISPR-CAS9 for remote control gene editing. The 2nd Trigger Method is for Remote Control Gene Editing. This method uses light to send a guided RNA method to CRISPR-CAS9 allowing the controller to grow Tumors, Cancer or Trigger Organ Failure.

John Hopkins - 2nd Method: Light-Activated 'CRISPR' Triggers Precision Gene Editing and Super-Fast DNA Repair

https://www.hopkinsmedicine.org/news/newsroom/news-releases/light-activated-crispr-triggers-precision-gene-editing-and-super-fast-dna-repair

CRISPR CONTROL PLATFORM

*CRISPR CHIP:

CRISPR-Chip Moves One Step Closer to the Genomics Market

https://www.technologynetworks.com/genomics/news/crispr-chip-moves-one-step-closer-to-the-genomics-market-323677

CRISPR-Chip: A New Biosensor for Electronic Detection of Target

https://www.synthego.com/blog/crispr-electronic-biosensor

CRISPR-Chip Launched as "Genome Sensor"

https://www.genengnews.com/topics/crispr-chip-launched-as-genome-sensor/

CRISPR-Chip - The World's First DNA Search Engine - Cardea Bio

https://cardeabio.com/crispr-chip/

CRISPR--Chip enables gene detection in less than 15 minutes

https://www.researchgate.net/figure/CRISPR-Chip-enables-gene-detection-in-less-than-15min-CRISPR-Chip-exploits-the_fig1_331991689

Genome Sensor - Google a Genome Using CRISPR-Chip | Nanosens

https://www.nanosens.com/genome-sensor/

CRISPR-based diagnostic chips perform thousands of tests

https://news.mit.edu/2020/crispr-diagnostic-chips-test-viruses-0429

Futuristic CRISPR-based biosensing in the cloud and internet of things

https://link.springer.com/article/10.1007/s11042-020-09010-5

CRISPR-Chip Detects Genetic Mutations in Minutes | Wearable

https://www.wearable-technologies.com/2019/04/new-crispr-chip-detects-genetic-mutations-in-minutes/

Ultra-sensitive and high-throughput CRISPR-powered COVID-19

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245202/

*FDA - ROLE IN THIS:

FDA Nears Approval for Injectable Bio-Chip Implants for COVID Detection

http://tapnewswire.com/2020/08/fda-nears-approval-of-injectable-biochip-implants-for-covid-detection-linked-to-computers/

FDA approves implantable chip to access medical records - NCBI

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC526112/

*MIT & HARVARD USE CRISPR TO EDIT DNA GELS FOR DIAGNOSTIC DEVICES

MIT & Harvard use CRISPR to "edit" DNA gels for diagnostic devices:

https://newatlas.com/science/crispr-gels-diagnostic-devices/

CRISPR - TARGETING SYSTEM

*FRIENDLY FIRE - CHROMOSOMAL TARGETING SYSTEM:

Friendly Fire: Biological Functions and Consequences of Chromosomal Targeting by CRISPR-Cas Systems

https://jb.asm.org/content/198/10/1481

*TARGET IDENTIFICATION & DRUG DISCOVERY:

Target Identification and Drug Discovery with CRISPR | Biocompare

http://www.biocompare.com/Editorial-Articles/361331-Target-Identification-and-Drug-Discovery-with-CRISPR/

*SIGMA ALDRICH CONTRIBUTION:

RNA APPLICATIONS

RNA DIRECTED NUCLEASE:

Gene Editing With CRISPR/Cas9 RNA-Directed Nuclease.

https://www.sigmaaldrich.com/catalog/papers/28254804

TRACING NUCLEASE BARCODING:

Quantitative Analysis of Synthetic Cell Lineage Tracing Using Nuclease Barcoding.

https://www.sigmaaldrich.com/catalog/papers/28264564

MICRO-RNA RESPONSIVE CRISPR-CAS9 ON / OFF SWITCH:

Cell-type-specific genome editing with a microRNA-responsive CRISPR-Cas9 switch.

https://www.sigmaaldrich.com/catalog/papers/28525578

AAV MEDIATED & RNA GUIDED CRISPR-CAS9 SYSTEM:

The AAV-mediated and RNA-guided CRISPR/Cas9 system for gene therapy of DMD and BMD.

https://www.sigmaaldrich.com/catalog/papers/28390761

RNA ACTIVATION INDEPENDANT DNA TARGETING OF TYPE 3 CRISPR-CAS SYSTEM:

RNA activation-independent DNA targeting of the Type III CRISPR-Cas system by a Csm complex.

https://www.sigmaaldrich.com/catalog/papers/28364023

STRUCTURAL BASIS FOR GUIDE RNA PROCESSING & SEED DEPENDENT DNA TARGETING SYSTEM USING CAS12A:

Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a.

https://www.sigmaaldrich.com/catalog/papers/28431230

CRISPR-CAS9 MICRO-INJECTION SYSTEM:

CRISPR/Cas9 microinjection in oocytes disables pancreas development in sheep

https://www.researchgate.net/publication/321640405_CRISPRCas9_microinjection_in_oocytes_disables_pancreas_development_in_sheep

DNA TARGETING MOLECULES:

Programmable type III-A CRISPR-Cas DNA targeting modules.

https://www.sigmaaldrich.com/catalog/papers/28441427

STRATEGY FOR ACTIVATION OF SILENT GENE CLUSTERS:

CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters.

https://www.sigmaaldrich.com/catalog/papers/28398287

CELL PENETRATING PEPTIDES SYSTEM:

Cell-penetrating peptides (CPPs): From delivery of nucleic acids and antigens to transduction of engineered nucleases for application in transgenesis.

https://www.sigmaaldrich.com/catalog/papers/28479163

GENE REGULATORY ELEMENTS SYSTEM:

Gene Regulatory Elements, Major Drivers of Human Disease.

https://www.sigmaaldrich.com/catalog/papers/28399667

APPLICATIONS OF CRISPR GENE EDITING TECHNOLOGY IN DRUG TARGET IDENTIFICATION & VALIDATION SYSTEM:

Applications of CRISPR genome editing technology in drug target identification and validation

https://www.sigmaaldrich.com/catalog/papers/28388235

DELIVERY TECHNOLOGIES FOR GENE EDITING:

Delivery technologies for genome editing.

https://www.sigmaaldrich.com/catalog/papers/28337020

CRISPR TRACKING SYSTEM

Tracking Multiple Genomic Elements Using Correlative CRISPR Imaging and Sequential DNAxa0FISH.

https://www.sigmaaldrich.com/catalog/papers/28355536

EDITING THE CENTRAL NERVOUS SYSTEM WITH CRISPR-CAS9

Editing the Central Nervous System Through CRISPR/Cas9 Systems

https://www.frontiersin.org/articles/10.3389/fnmol.2019.00110/full

USING CRISPR-CAS13

Treating Influenza and SARS-CoV-2 via mRNA-encoded Cas13a

https://www.biorxiv.org/content/10.1101/2020.04.24.060418v1

CRISPR/Cas13a Powered Portable Electrochemiluminescence (Light)

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.201903661

DATA MINING USING CRISPR

Data Mining by Pluralistic Approach on CRISPR Gene Editing in

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6632506/

CRISPR mines bacterial genome for hidden pharmaceutical

https://www.sciencedaily.com/releases/2017/04/170411085819.htm

CRISPR-Cas9/Cas12a biotechnology and application in bacteria

https://www.sciencedirect.com/science/article/pii/S2405805X18300565

ALTERNATE GENE EDITING USING CAST INSTEAD OF CUTTING

*MIT - CREATED CAST METHOD:

MIT’s CRISPR gene editing alternative inserts new DNA without cutting.

The team isolated the enzyme Cas12k from two species of cyanobacteria and manipulated them into jumping to set targets in the genome, then inserting new DNA sequences without having to cut anything. The new system was named CRISPR-Associated Transposase or CAST for short.

https://www.fanaticalfuturist.com/2019/07/mits-crispr-gene-editing-alternative-inserts-new-dna-without-cutting/

HARVARD CONTRIBUTIONS

Researchers ID molecules that rein in CRISPR - Harvard Gazette

https://news.harvard.edu/gazette/story/2019/05/brigham-broad-institute-researchers-id-molecules-that-rein-in-crispr-systems/

CRISPR CAS APPLICATIONS

The CRISPR-Cas immune system: Biology, mechanisms and applications

https://www.sciencedirect.com/science/article/pii/S0300908415001042

Anti-CRISPR proteins encoded by archaeal lytic viruses inhibit subtype I-D immunity:

https://europepmc.org/article/med/29507349

*TRACKING CRISPR FOOTPRINTS

Tracking CRISPR’s Footprints:

https://experiments.springernature.com/articles/10.1007/978-1-4939-9170-9_2

Positive Identification of DNA Cleavage by CRISPR-Cas9 Using Pyrene Excimer Fluorescence to Detect a Subnanometer Structural Change:

https://pubs.acs.org/doi/10.1021/acs.jpclett.9b01913

Annotation and Classification of CRISPR-Cas Systems:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5901762/

Integrated DNA Technologies, Inc. - All in One CRISPR Library with DNA sequences:

https://www.idtdna.com/pages

*CRISP-ID WEB APPLICATION - ALLOWS DETECTION OF THE EXACT INDEL SIZE & LOCATION OF TARGETED REGION

CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing:

https://www.nature.com/articles/srep28973

CRISP-ID: Detecting CRISPR mediated indels by Sanger sequencing. CRISP-ID is a web application that allows the detection of the exact indel size and location of a CRISPR-Cas9 targeted region, based on direct Sanger sequencing.

http://crispid.gbiomed.kuleuven.be

*CRISPR CAS INDENTIFIER SYSTEM:

CRISPRcasIdentifier: Machine learning for accurate identification and classification of CRISPR-Cas systems:

https://academic.oup.com/gigascience/article/9/6/giaa062/5858408

CRISPR GENE DRIVE SYSTEMS

Current CRISPR gene drive systems are likely to be highly invasive in wild populations

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014726/

CRISPR INTERATIONS WITH HYDROGEL & SMART MATERIALS

CRISPR Propels a Smart Hydrogel

https://science.sciencemag.org/content/365/6455/754

Programmable CRISPR-responsive Smart Materials

https://science.sciencemag.org/content/365/6455/780

Shapeshifting hydrogels with CRISPR inside rearrange on command and interface with our bodies. Programmable CRISPR-responsive smart materials, like DNA hydrogels, are much more likely to become game-changers in our lifetimes

https://massivesci.com/notes/crispr-engineered-dna-hydrogels-can-enhance-smart-biomaterials/

Crispr Deployed For Programmable Smart Hydrogels

https://sciencebusiness.technewslit.com/?p=37234

Harvard - Biomaterials smarten up with CRISPR

https://wyss.harvard.edu/news/biomaterials-smarten-up-with-crispr/

Material solutions for delivery of CRISPR/Cas-based genome editing tools: Current status and future outlook

https://www.sciencedirect.com/science/article/abs/pii/S136970211830854X

Using CRISPR to program gels with new functions:

Smart materials change properties in response to specific DNA sequences; could be used in a variety of devices.

https://news.mit.edu/2019/crispr-edit-materials-gels-0822

PROGRAMMABLE CRISPR-RESPONSIVE SMART MATERIALS

Applications

This technology is a programmable, stimuli-responsive biomaterial with broad applications in medicine and biotechnology, including tissue engineering, molecular diagnostics, environmental monitoring, bioelectronics, and microfluidics.

https://tlo.mit.edu/technologies/programmable-crispr-responsive-smart-materials

Biomaterials Smarten Up with CRISPR

https://www.nanowerk.com/news2/biotech/newsid=53454.php

Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets

https://pubs.acs.org/doi/10.1021/jacs.9b09211

Sigma Aldrich - Nano and Microfabricated Hydrogels for Regenerative Engineering

https://www.sigmaaldrich.com/life-science/learning-center/customer-education/hydrogels-for-regenerative-engineering-webinar.html

DNA Triggers Shape-Shifting in Hydrogels, Opening a New Way to Make ‘Soft Robots’

https://releases.jhu.edu/2017/09/15/dna-triggers-shape-shifting-in-hydrogels-opening-a-new-way-to-make-soft-robots/

News Release Archive - 2017 - Johns Hopkins Medicine:

https://www.hopkinsmedicine.org/news/media/releases/2017

Characterizing exogenous mRNA delivery, trafficking, cytoplasmic release and RNA--protein correlations at the level of single cells:

https://academic.oup.com/nar/article/45/12/e113/3760188

Berkley - Sprinkling of Neural Dust opens door to electroceuticals:

https://news.berkeley.edu/2016/08/03/sprinkling-of-neural-dust-opens-door-to-electroceuticals/

GENE DRIVES

DARPA INVOLVEMENT:

DARPA / University of California - DARPA funds UC gene drive research against mosquito-borne diseases:

https://www.sandiegouniontribune.com/business/biotech/sd-me-darpa-ucsd-20170719-story.html

DARPA - Invests 100 Million Dollars for Gene Extinction Technologies:

https://www.theguardian.com/science/2017/dec/04/us-military-agency-invests-100m-in-genetic-extinction-technologies

WORLD HEALTH ORGANIZATION & BILL & MELINDA GATES FOUNDATION:

WHO / Bill & Melinda Gates Foundation - The Bill and Melinda Gates foundation has recently donated over $75 million to fund Gene Drive mosquito research by Target Malaria , a consortium that aims to develop technology for malaria control.

http://science-union.org/articlelist/2019/9/20/gene-drive-mosquitoes-ethics-environment-and-efficacy

JOHN HOPKINS INVOLVEMENT:

John Hopkins & Bloomberg (Both Attended Event 201) - New Report from Johns Hopkins Center for Health Security: Gene Drives: Pursuing Opportunities, Minimizing Risk

https://www.centerforhealthsecurity.org/news/center-news/2020-05-20-gene-drives-report.html

POPULATION CONTROL:

Progress Towards Engineering Gene Drives for Population Control

https://jeb.biologists.org/content/223/Suppl_1/jeb208181

Military Revealed Top Funder of Gene Drives; Gates Foundation paid $1.6 million to influence UN on Gene Drives

https://foe.org/news/military-revealed-top-funder-gene-drives-gates-foundation-paid-1-6-million-influence-un-gene-drives/

WHAT ARE GENE DRIVES:

GENE DRIVES (ALTER AN ENTIRE SPECIES FROM WITHIN) -- PROGRAMMABLE POPULATION CONTROL

Gene Drives are a reality with a new study showing efficient ERADICATION of malaria carrying mosquito populations. Gene Drive applications for most conservation needs require a gene drive working in mammals and more specifically in rats. CRISPR-Cas9 Gene Drives don’t work well in mammals yet, which pushes gene drive applications for conservation into the future. Applying gene drives for conservation purposes also needs a Safety Mechanism, like a Daisy Drive System, that doesn’t allow the drive to spread beyond the intended population.

Powerful 'Gene Drive' Can Quickly Change An Entire Species

https://www.npr.org/sections/health-shots/2015/11/05/451216596/powerful-gene-drive-can-quickly-change-an-entire-species

What's a gene drive?

https://youtu.be/KgvhUPiDdq8

A gene drive is a radical new way of re-engineering the genetic makeup of an entire species - including harmful insects like the mosquito. But as the technology steams head, fueled by powerful gene editing techniques coming out of synthetic biology like CRISPR Cas9, what are the risks, and how can we ensure that it is used safely and responsibly without causing harm?

How gene drive technology works (video) The Wyss Institute: https://youtu.be/G1L0G00nCM8

This animation explains how an emerging technology called "gene drives" may be used to potentially spread particular genomic alterations through targeted wild populations over many generations. It uses mosquitoes as an example of a target species --- and illustrates how the versatile genome editing tool called CRISPR makes it possible.

Gene drive' mosquitoes engineered to fight malaria. Nature News, November 23 2015.

https://www.nature.com/news/gene-drive-mosquitoes-engineered-to-fight-malaria-1.18858

World Economic Forum (WEF): How gene editing is changing the world

https://www.weforum.org/agenda/2015/11/how-gene-editing-is-changing-the-world/

On the reversibility of gene drives. The Scientist:

https://www.the-scientist.com/opinion/opinion-on-the-irreversibility-of-gene-drives-36842

Regulating Gene Drives -- Science Magazine, 8 August 2014:

https://science.sciencemag.org/content/sci/345/6197/626.full.pdf

Gene Drive -- This could be the next weapon of mass destruction. Quartz (look beyond the hyperbolic headline!):

https://qz.com/554337/this-could-be-the-next-weapon-of-mass-destruction/

Breakthrough DNA Editor Borne of Bacteria. Carl Zimmer, Quanta Magazine. Feb 26 2015.

https://www.quantamagazine.org/crispr-natural-history-in-bacteria-20150206

Safety upgrade found for gene-editing technique -- Nature News:

https://www.nature.com/news/safety-upgrade-found-for-gene-editing-technique-1.18799

CRISPR Gene Drive (Complete guide 2019)

https://youtu.be/_zri19Vp34E

Genome Editing with CRISPR-Cas9

https://youtu.be/2pp17E4E-O8

Gene Drives are a new biotechnology development that allow humans the unprecedented capability to profoundly alter or even drive to extinction entire populations or whole species of organisms.

http://www.synbiowatch.org/gene-drives/

Gene drives are genes that do not adhere to Mendelian genetics during sexual reproduction and are inherited at higher rates in progeny, and can therefore spread very quickly through a population.

https://www.sciencedirect.com/topics/medicine-and-dentistry/gene-drive

Gene drives in our future: Challenges of and opportunities for using a self-sustaining technology in pest and vector management

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6069294/

How self-propagating CRISPR-based gene drives can be used to spread genetic alterations through wild populations, potentially impacting all organisms of the target species.

https://www.ibiology.org/genetics-and-gene-regulation/gene-drive/

A transcomplementing gene drive provides a flexible platform for laboratory investigation and potential field deployment

https://www.nature.com/articles/s41467-019-13977-7

Gene drives for schistosomiasis transmission control

https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0007833

Neofunctionalization of Duplicated P450 Genes Drives

https://www.cell.com/current-biology/abstract/S0960-9822(17)31542-7

Concerning RNA-guided gene drives for the alteration of wild - NCBI

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117217/

Gene Drives - Sculpting Evolution

http://www.sculptingevolution.org/genedrives

GENE DRIVES - TARGETING SYSTEM

*DAISY CHAIN GENE DRIVES - TARGETING GROUPS WITHIN A POPULATION

What are Daisy Chain Gene Drives?

https://youtu.be/n71fcuFtbgQ

Daisy-chain Gene Drives for the Alteration of Local Populations

https://www.pnas.org/content/116/17/8275

DAISY DRIVE SYSTEM (Allows them to Selectively target certain people based on criteria)

The problem with current CRISPR-based gene drive systems is that they can spread indefinitely---potentially affecting every population of the target species throughout the world.

Control & Target Specific Genes

- DNA to count and limit gene drive spreading to a pre-programmed number of generations.

GENE DRIVES - GUIDANCE SYSTEM

*RNA GUIDED GENE DRIVES:

President & Fellows of Harvard College - Google Patent - RNA-Guided Gene Drives:

https://patents.google.com/patent/WO2015105928A1/en

Bioethics in Brief -- CRISPR, Gene Drives and Genetically-modified Mosquitoes - Engineering Extinction: CRISPR, Gene Drives and Genetically-Modified Mosquitoes

https://www.biotech.iastate.edu/bib-crispr/

CRISPR Science: DNA, RNA, and Gene Editing

Gene Silencing by microRNAs

MicroRNAs are a class of small, non-coding RNA molecules that regulate gene expression and have a big impact on many biological processes. This medical 3D animation shows the biogenesis and function of microRNAs within the cell. The goal of the project was to arouse interest by presenting the topic of gene silencing in a simplified and visually appealing way.

RNAi: Gene Regulation via miRNAs & siRNAs

This is important

mRNA vaccines --- a new era in vaccinology

https://www.nature.com/articles/nrd.2017.243

University of Cambridge - Introduction to RNA Vaccines

https://www.phgfoundation.org/briefing/rna-vaccines

mRNA as a Transformative Technology for Vaccine Development to Control Infectious Diseases

https://www.sciencedirect.com/science/article/pii/S1525001619300413

Dual Luciferase Gene Reporter Assays to Study miRNA Function

https://experiments.springernature.com/articles/10.1007/978-1-4939-2547-6_17

Self-Amplifying mRNA Vaccines

https://www.sciencedirect.com/science/article/pii/S0065266014000066

microRNA Target Gene Luciferase Reporter Constructs

https://www.biocat.com/genomics/microrna/microrna-target-gene-luciferase-reporter-constructs

Delivering the Messenger: Advances in Technologies for Therapeutic mRNA Delivery

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453548/

mRNA as a Transformative Technology for Vaccine Development to Control Infectious Diseases

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453507/

A Single Administration of CRISPR/Cas9 LipidNanoparticles Achieves Robust and Persistent In Vivo Genome Editing

https://www.cell.com/cell-reports/pdf/S2211-1247(18)30182-7.pdf

*SIGMA ALDRICH*

CRISPR mRNAs, including Cas9 mRNA, is ready to use in transgenic applications. Must be used in conjunction with a purified guideRNA sequence to mediate a site specific double strand break in the DNA.

https://www.sigmaaldrich.com/catalog/product/sigma/cas9mrna?lang=en&region=US

GENETIC CONTROL

There are a couple options that can be considered for genetic control.

*ONE OPTION - GENE DRIVES:

One strategy is to use a gene drive to propagate a negative trait through a population, such as female sterility.

*ANOTHER OPTION RNA INTERFERENCE (RNAI):

Another strategy is to use RNA interference (RNAi) to selectively turn off critical genes of the target organism. This strategy takes advantage of the natural gene regulation mechanism of RNA-induced gene silencing. We target unique genetic sequences of zebra mussels that do not occur in other species for critical genes provide the instructions (silencing RNA) to turn off those genes. Because the target sequences do not occur in other species, it makes the control tool very specific.

Developing RNA Interference to Control Zebra Mussels: Introduction of RNA "Gene Silencing".

https://www.usgs.gov/centers/umesc/science/developing-rna-interference-control-zebra-mussels?qt-science_center_objects=0#qt-science_center_objects

Regulatory DNA / RNA / RNAi - Biology | Socratic

https://socratic.org/biology/signal-transduction-and-control-of-gene-expression/regulatory-dnarnarnai

The State of Knowledge of the Molecular Biology, Population - NCBI

https://www.ncbi.nlm.nih.gov/books/NBK379272/

*EXERCISE ACTIVATED NEUROTROPIC SIGNALING ON MRNA LEVELS OF DOWNSTREAM SIGNALING MOLECULES - THIS LEADS TO BILL GATES PATENTED CRYPTOCURRANCY:

The Effects of Voluntary Physical Exercise-Activated Neurotrophic Signaling in Rat Hippocampus on mRNA Levels of Downstream Signaling Molecules.

https://www.sigmaaldrich.com/catalog/papers/28439815

Regulatory DNA / RNA / RNAi - Biology | Socratic

https://socratic.org/biology/signal-transduction-and-control-of-gene-expression/regulatory-dna- -rna- -rnai

Nanotechnology for COVID-19:

Therapeutics and Vaccine Research

The role of nanotechnology is highly relevant to counter this “virus” nano enemy. Nano intervention is discussed in terms of designing effective nanocarriers to counter the conventional limitations of antiviral and biological therapeutics. This strategy directs the safe and effective delivery of available therapeutic options using engineered nanocarriers, blocking the initial interactions of viral spike glycoprotein with host cell surface receptors, and disruption of virion construction. Controlling and eliminating the spread and reoccurrence of this pandemic demands a safe and effective vaccine strategy.

https://pubs.acs.org/doi/10.1021/acsnano.0c04006

A Nanomaterial Path Forward for COVID-19 Vaccine Development

From mRNA vaccines entering clinical trials, to peptide-based vaccines and using molecular farming to scale vaccine production, the COVID-19 pandemic is pushing new and emerging nanotechnologies into the frontlines and the headlines. Nanoengineers detail the current approaches to COVID-19 vaccine development, and highlight how nanotechnology has enabled these advances, in a review article.

https://www.sciencedaily.com/releases/2020/07/200715095500.htm

DARPA

Bill Gates Partners DARPA For New DNA Nanotech COVID-19 Vaccine

https://www.mirrorreview.com/bill-gates-partners-darpa-for-covid-19-vaccine/

DARPA invests $7.5 million into Profusa’s tiny tech-packed implantable biosensor

July 17, 2016

https://www.digitaltrends.com/cool-tech/darpa-awards-profusa-grant-for-implantable-biosensor/

DARPA - Biological Technologies Office (BTO) Dr. Rohit Chitale Worked with World Health Organization (WHO), CDC and the Bill & Melinda Gates Foundation

https://www.darpa.mil/staff/dr-rohit-chitale

DARPA COVID 19 PLAN

https://www.darpa.mil/work-with-us/covid-19

Epigenetic CHaracterization and Observation (ECHO)

https://www.darpa.mil/program/epigenetic-characterization-and-observation

Prometheus

https://www.darpa.mil/program/prometheus

DARPA-funded microchip technology optimizes convalescent plasma therapy for COVID-19 patients

https://www.newswise.com/coronavirus/darpa-funded-microchip-technology-optimizes-convalescent-plasma-therapy-for-covid-19-patients/?page=2&search%5bstatus%5d=3&search%5bsort%5d=date+desc&search%5bchannel_id%5d=6546&category=)

Friend or Foe

https://www.darpa.mil/program/friend-or-foe

SIGMA+

https://www.darpa.mil/program/sigma-plus

PReemptive Expression of Protective Alleles and Response Elements (PREPARE)

https://www.darpa.mil/program/preemptive-expression-of-protective-alleles-and-response-elements

DARPA - The Right Chemistry, Fast: Employing AI and Automation to Map Out and Make Molecules

https://www.darpa.mil/news-events/2018-06-19

DARPA - HYDROGEL

New DARPA injectable solution enables government tracking and monitoring of recipients.

https://z3news.com/w/darpa-injectable-solution-enables-government-tracking-monitoring-recipients/

DARPA Targeted Neuroplasticity Training (TNT)

https://www.darpa.mil/program/targeted-neuroplasticity-training

DARPA TNT Researchers Set Out to Advance Pace and Effectiveness of Cognitive Skills Training

https://www.darpa.mil/news-events/2017-04-26

Darpa Goal: Psychic Doctors

https://www.wired.com/2007/10/darpas-nose-for/

DARPA In Vivo Nanoplatforms (IVN)

https://www.darpa.mil/program/in-vivo-nanoplatforms

DARPA Neural Engineering System Design (NESD)

https://www.darpa.mil/program/neural-engineering-system-design

DARPA Towards a High-Resolution, Implantable Neural Interface

https://www.darpa.mil/news-events/2017-07-10

DARPA Is Planning to Hack the Human Brain to Let Us “Upload” Skills

https://futurism.com/darpa-is-planning-to-hack-the-human-brain-to-let-us-upload-skills

DARPA's New Biotech Division Wants To Create A Transhuman Future

https://io9.gizmodo.com/darpas-new-biotech-division-wants-to-create-a-transhum-1556857603

DARPA's Blue Angel - Pentagon prepares millions of vaccines against future global flu

https://www.rt.com/usa/future-vaccine-darpa-research-255/

DARPA Effort Speeds Biothreat Response - DOD News

https://archive.defense.gov/News/NewsArticle.aspx?ID=61520

DARPA effort speeds bio-threat response - Army News

https://www.army.mil/article/47617/darpa_effort_speeds_bio_threat_response

DARPA’s Blue Angel Project 2014

https://www.pharmaceutical-technology.com/features/featureblue-angel-darpas-vaccine-manufacturing-challenge-4171658/

DARPA’s Blue Angel -- Pentagon Prepares Millions of Vaccines Against Future Global Flu - July 28th 2012

https://www.globalresearch.ca/darpa-s-blue-angel-pentagon-prepares-millions-of-vaccines-against-future-global-flu/32141

After the Lockdown: A Global Coronavirus Vaccination Program…

https://www.globalresearch.ca/after-the-lockdown-a-global-coronavirus-vaccination-program/5706547?utm_campaign=magnet&utm_source=article_page&utm_medium=related_articles

Hydrogel Machines - MIT

http://zhao.mit.edu/wp-content/uploads/2020/06/125.pdf

Hydrogels and polymers as components of a lab on a chip

https://www.sciencedirect.com/science/article/pii/B9780444511003500012

NATIONAL INSTITUTE OF HEALTH (NIH) CONNECTION

NIH, DARPA and FDA collaborate to develop cutting-edge technologies to predict drug safety

Friday, September 16, 2011

https://www.nih.gov/news-events/news-releases/nih-darpa-fda-collaborate-develop-cutting-edge-technologies-predict-drug-safety

DARPA and NIH to fund ‘human body on a chip’ research

July 24, 2012

https://news.mit.edu/2012/human-body-on-a-chip-research-funding-0724

DARPA and the Brain Initiative

https://www.darpa.mil/program/our-research/darpa-and-the-brain-initiative

Recent IARPA, DARPA, NIH RFPs Require Computational Expertise

https://www.cccblog.org/2011/09/22/recent-iarpa-darpa-nih-rfps-require-computational-expertise/

National Institutes of Health (NIH)

Brain Research through Advancing Innovative Neurotechnologies (BRAIN) Working Group Report to the Advisory Committee to the Director, NIH June 5, 2014

https://braininitiative.nih.gov/strategic-planning/brain-2025-report

The BRAIN Initiative 2.0: From Cells to Circuits, Toward Cures

https://braininitiative.nih.gov/strategic-planning/acd-working-groups/brain-initiative-20-cells-circuits-toward-cures

BRAIN CONNECTION

DARPA-Funded Research Offers Faster, Better Views of Entire Brain

https://www.afcea.org/content/darpa-funded-research-offers-faster-better-views-entire-brain

The Evolution of Neuroprosthetic Interfaces

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541680/

Multifunctional Nanobiomaterials for Neural Interfaces - BRAIN INTERFACE

https://deepblue.lib.umich.edu/bitstream/handle/2027.42/61888/573_ftp.pdf;sequence=1

WHAT MAKES UP HYDROGEL?

Hydrogels: Methods of Preparation, Characterisation and Applications | IntechOpen

https://www.intechopen.com/books/progress-in-molecular-and-environmental-bioengineering-from-analysis-and-modeling-to-technology-applications/hydrogels-methods-of-preparation-characterisation-and-applications

Biologically Derived Soft Conducting Hydrogels Using Heparin-Doped Polymer Networks

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4046800/

Hydrogels and polymers as components of a lab on a chip

https://www.sciencedirect.com/science/article/pii/B9780444511003500012

Nano-structured Hydrogels

https://www.sciencedirect.com/science/article/pii/B9781455731596000109

Nano-structured smart hydrogels with rapid response and high elasticity

https://www.nature.com/articles/ncomms3226

Nano-structured smart hydrogels with rapid response and high elasticity

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3731657/

Next-generation probes, particles, and proteins for neural interfacing

https://advances.sciencemag.org/content/3/6/e1601649.full

Crafting Nanostructured Neural Interfaces with Hydrogel Particles

https://trace.tennessee.edu/utk_graddiss/4704/

Hierarchical nanostructured conducting polymer hydrogel with high electrochemical activity - BIO-ELECTRICONICS

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3386113/

Multifunctional Nanobiomaterials for Neural Interfaces - BRAIN INTERFACE

https://deepblue.lib.umich.edu/bitstream/handle/2027.42/61888/573_ftp.pdf;sequence=1

Nanoporous Gold as a Neural Interface Coating: Effects of Topography, Surface Chemistry, and Feature Size

https://pubs.acs.org/doi/10.1021/acsami.5b00410

Gold Nanoparticle/Polymer Nanocomposites:  Dispersion of Nanoparticles as a Function of Capping Agent Molecular Weight and Grafting Density

https://pubs.acs.org/doi/abs/10.1021/la047193e

Nanoparticle and Gelation Stabilized Functional Composites of an Ionic Salt in a Hydrophobic Polymer Matrix

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916421/

Nanocomposites of polymer and inorganic nanoparticles for optical and magnetic applications

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3215211/

The Hydrogels: What are Hydrogels?

http://2017.igem.org/Team:TU_Darmstadt/project/hydrogel

Continuous monitoring of interstitial tissue oxygen using subcutaneous oxygen microsensors: In vivo characterization in healthy volunteers

https://pubmed.ncbi.nlm.nih.gov/30742844/

Fabrication and Applications of Micro/Nanostructured Devices for Tissue Engineering

https://link.springer.com/article/10.1007/s40820-016-0103-7

Biomimetic Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications

http://www.gbv.de/dms/tib-ub-hannover/773809066.pdf

Hydrogel Bioelectronics

https://pubs.rsc.org/en/content/articlepdf/2019/cs/c8cs00595h

Researchers revolutionize brain-computer interfaces using silicon electronics

https://www.nanowerk.com/nanotechnology-news/newsid=47371.php

Bioinspired microcone-array-based living biointerfaces: enhancing the anti-inflammatory effect and neuronal network formation

https://www.nature.com/articles/s41378-020-0172-0

Biomedical Applications of DNA‐Based Hydrogels

https://onlinelibrary.wiley.com/doi/full/10.1002/adfm.201906253

Special Issue "Smart Hydrogels for Biomedical Applications"

https://www.mdpi.com/journal/polymers/special_issues/smart_hydrogel

A scalable peptide-GPCR language for engineering multicellular communication

https://www.nature.com/articles/s41467-018-07610-2

Discovery and design of self-assembling peptides - IMPORTANT - *THIS IS HOW HYDROGEL INSTANTLY STARTS TO BUILD ON ITSELF TO CONNECT YOU TO AI*

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665798/

Hydrogels Derived from Central Nervous System Extracellular Matrix

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3512573/

Injectable hydrogels for central nervoussystem therapy

https://shoichetlab.utoronto.ca/wp-content/uploads/2018/07/injectable-hydrogels-for-central-nervous-system-therapy.pdf

Reliable Central Nervous System Interfaces

https://www.darpa.mil/work-with-us/reliable-central-nervous-system-interfaces

NIH - Peptide Hydrogels for Rate-Controlled Delivery of Therapeutics

https://www.ott.nih.gov/technology/e-188-2017

Application of Metal Nanoparticle--Hydrogel Composites in Tissue Regeneration

https://www.mdpi.com/2306-5354/6/1/17/pdf

BlackRock Owns All 3 Vaccine Companies

Moderna & AstraZeneca - Same investors BlackRock

https://www.marketwatch.com/story/moderna-ipo-5-things-to-know-about-what-could-be-the-largest-biotech-ipo-in-history-2018-12-05

Gilead Sciences - Remedivir - Same investors BlackRock

https://fintel.io/so/us/gild/blackrock

HSBC signs deal to use BlackRock's 'Aladdin' software worldwide

Published: Mar 21, 2019 11:13AM EDT

https://www.nasdaq.com/articles/hsbc-signs-deal-use-blackrocks-aladdin-software-worldwide-2019-03-21

MRNA VACCINES - CAN THEY GENETICALLY MODIFY YOU IF SO DOES THAT MEAN YOU CAN BE OWNED?

Technically, Yes! Once genetically modified you are no longer human you are considered GMO

Patentability of Genetically Modified Organisms (GMO's)

https://www.grin.com/document/13628

Biotechnology Patents and Genetically Modified Organisms

https://www.taconic.com/taconic-insights/model-generation-solutions/biotechnology-patents-and-gmos.html#

Next Tracking Your Live Inventory

Barcoding the cell's nucleus

https://www.nature.com/articles/nrg.2017.11

Cellular barcoding is a technique in which individual cells or cell populations are genetically labeled with unique nucleic acid sequences, termed barcodes, so that they can be monitored through space and time by Next Generation Sequencing.

https://nucleusbiotech.com/produkt-kategorie/cell-barcoding/

CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics

https://genomebiology.biomedcentral.com/articles/10.1186/s13059-019-1699-y

Our Feature Barcoding technology, which is currently compatible with our Single Cells. Prior to Gilead, Dr. Suliman was an investment banker with Lehman Brothers (Connection to Gilead Sciences)

https://www.sec.gov/Archives/edgar/data/1770787/000119312519224368/d737378ds1.htm

METHOD THAT WILL BE USED TO TRACK CELL BARCODING

Fluorescent cell barcoding for multiplex flow cytometry

https://pubmed.ncbi.nlm.nih.gov/21207359/

Optimization and standardization of fluorescent cell barcoding for multiplexed flow cytometric phenotyping

https://pubmed.ncbi.nlm.nih.gov/28692789/

Mass cytometry: a highly multiplexed single-cell technology for advancing drug development

https://pubmed.ncbi.nlm.nih.gov/25349123/

A Universal Live Cell Barcoding-Platform for Multiplexed Human Single Cell Analysis

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6050312/

DNA Barcodes Make for Better Single-Cell Analysis

https://directorsblog.nih.gov/2018/04/16/dna-barcodes-make-for-better-single-cell-analysis/

Recording every cell’s history in real-time with evolving genetic barcodes - HARVARD

https://wyss.harvard.edu/news/recording-every-cells-history-in-real-time-with-evolving-genetic-barcodes/

Developmental barcoding of whole mouse via homing CRISPR

https://science.sciencemag.org/content/361/6405/eaat9804

Developmental barcoding of whole mouse via homing CRISPR

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6139672/

Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPRCas9 barcodes by scGESTALT

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6279253/

Whole-organism lineage tracing by combinatorial and cumulative genome editing

https://pubmed.ncbi.nlm.nih.gov/27229144/

GESTALT, the CRISPR/Cas9 lineage tracing approach from the Shendure lab and Schier lab

Shendure Lab (Neural Network Architecture Tuning)

https://krishna.gs.washington.edu/

Schier Lab (Morphogens & Gene Regulatory Networks / Neuropeptides & Neural Circuits Regulates Behavior)

https://schierlab.biozentrum.unibas.ch/

BLACKROCK MICROSYSTEMS & DARPA

Blackrock products are continually chosen for the highest profile human research projects such as BrainGate, DARPA Revolutionizing Prosthetics, DARPA Restoring Active Memory and DARPA HAPTIX.

Blackrock Microsystems Human Neuroscience Research Capabilities

https://www.blackrockmicro.com/neuroscience-research-projects/human-research-systems/

BRAINGATE

Blackrock Microsystems and actually made a brain implant which can remotely control various devices in your home. (Brain Signal Management)

https://www.blackrockmicro.com/control-your-home-with-a-brain-implant/

Neural Signal Processor (Hardware)

The NeuroPort™ system is a highly configurable, easy to use, multichannel data acquisition system used for recording and monitoring brain electrical activity.

https://www.blackrockmicro.com/neuroscience-research-products/neural-data-acquisition-systems/neuroport-daq-system/

DARPA HAPTIX

Hand Proprioception and Touch Interfaces - HAPTIX (Touch Sensory & Signal Reciver)

https://www.darpa.mil/program/hand-proprioception-and-touch-interfaces

DARPA Revolutionizing Prosthetics (Modular Limbs & Signal Reciver)

https://www.darpa.mil/program/revolutionizing-prosthetics

United Allied States

https://uas.govt.agency/

WORLD ECONOMIC FORUM ROLE

DNA Storage scientist Nick Goldman issued the Bitcoin Challenge at World Economic Forum meeting in Davos, January 2015.

https://www.ebi.ac.uk/about/news/press-releases/belgian-phd-student-decodes-dna-wins-bitcoin

DNA storage: a new method for storing digital information

https://www.ebi.ac.uk/research/goldman/dna-storage

Fourth Industrial Revolution for the Earth Series

Building Block(chain)s for a Better Planet

http://www3.weforum.org/docs/WEF_Building-Blockchains.pdf

WEF launches blockchain traceability platform for sustainability

https://www.ledgerinsights.com/wef-blockchain-traceability-sustainability/

TextileGenesis™ is a pioneering traceability platform custom built for the apparel ecosystem

https://textilegenesis.com/

World Economic Forum - Continuous Interconnected Supply Chain Using Blockchain & Internet-of-Things in Supply Chain Traceability

https://www2.deloitte.com/content/dam/Deloitte/lu/Documents/technology/lu-blockchain-internet-things-supply-chain-traceability.pdf

Several large technologyfirms, such as IBM, Intel, and Microsoft, are heavilyinvested in blockchain technology development, and theWorld Economic Forum estimates that, by 2025, 10% ofthe global gross domestic product will be stored on block-chain technology

https://bmcmedicine.biomedcentral.com/track/pdf/10.1186/s12916-019-1296-7

VIDEO EXPLAINS THE SCIENCE POSTED ABOVE

DARPA Targeted Neuroplasticity Training (TNT)

https://www.darpa.mil/program/targeted-neuroplasticity-training

DARPA TNT Researchers Set Out to Advance Pace and Effectiveness of Cognitive Skills Training

https://www.darpa.mil/news-events/2017-04-26

Darpa Goal: Psychic Doctors

https://www.wired.com/2007/10/darpas-nose-for/

DARPA In Vivo Nanoplatforms (IVN)

https://www.darpa.mil/program/in-vivo-nanoplatforms

DARPA Neural Engineering System Design (NESD)

https://www.darpa.mil/program/neural-engineering-system-design

DARPA Towards a High-Resolution, Implantable Neural Interface

https://www.darpa.mil/news-events/2017-07-10

DARPA Is Planning to Hack the Human Brain to Let Us “Upload” Skills

https://futurism.com/darpa-is-planning-to-hack-the-human-brain-to-let-us-upload-skills