/random/ - random

This is simply a dump of research found.

Figure MG-1. TRPV1-IONP system. The IONP is covalently coated in anti-His6 antibodies allowing it to bind to the His6-tag on the channel. When subjected to RF waves, the nanoparticle activates the channel and causes it to open. This results in calcium ion flux into the cell and the subsequent expression of a calcium-promoted gene. [We would like to acknowledge UltraFlex Power Technologies for the custom RF induction system used to generate the RF waves in the in vitro and in vivo studies described herein.]

Figure in above post.

>>59511

Figure MG-4. Efficacy of TRPV1-ferritin system in mice. (a) RF treatment of mice implanted with mesenchymal stem cells (MSCs) expressing the TRPV1-ferritin system (Figure 3) and an analogous TRPV1-myrferritin system (not shown) were found to reduce blood glucose compared with control mice (* P < 0.05). (b) Total blood glucose reduced by TRPV1-ferritin and TRPV1-myrferritin systems over 60 min of RF treatment and 60 min following RF treatment as compared to the control (* P < 0.05). TRPV1-myrferritin system used myristoylated ferritin resulting in random integration of ferritin into the cell membrane, thus placing ferritin with varying proximity to the TRPV1 channels distributed throughout the membrane. In comparison to the αGFP-TRPV1 direct conjugation to the GFP-ferritin, data shows that close proximity has significant impact in improving response to RF stimulation. Data shown as Mean ± S.E.M.

Using a similar approach, we further targeted and temporally regulated neural modulation to determine the physiological roles of specific neural populations or circuits. We induced neuronal activation remotely using either RF or magnetic fields via Cre-dependent expression of the aforementioned GFP-tagged ferritin fusion protein tethered to the αGFP-TRPV1 fusion protein3. Neuronal inhibition via the same stimuli is achieved by mutating the TRPV1 pore, rendering the channel chloride-permeable. These constructs were targeted to glucose-sensing neurons in the ventromedial hypothalamus in glucokinase–Cre (GK-Cre) mice, which express Cre in glucose-sensing neurons. Acute activation, via Ca2+ flux, of glucose-sensing neurons in this region was found to increase plasma glucose and glucagon levels, lower insulin levels, and stimulate feeding (Figure MG-5a). Conversely, inhibition via Cl- flux through the mutant TRPV1 resulted in reduced blood glucose levels, increased insulin levels, and the suppression of feeding (Figure MG-5b). These results suggest that pancreatic hormones function as an effector mechanism of central nervous system circuits controlling blood glucose and behavior. Our method obviates the need for permanent implants and could potentially be applied to study other neural processes or used to regulate other, even dispersed, cell types.

Now for a brief break:

The frequency bands for 5G networks come in two sets. Frequency range 1 is from 450 MHz to 6 GHz. Frequency range 2 is from 24.25 GHz to 52.6 GHz. To share frequencies used by LTE and 5G networks, frequency- and time division duplexing can be used.

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Near-infrared light generally refers to light within the wavenumber range of 12,500 to 4,000 cm-1 (wavelengths from 800 to 2,500 nm) (see Fig. 1). Absorption of near-infrared light, like that of mid-infrared light, is based on the vibration of the material.

IR frequencies range from about 300 gigahertz (GHz) up to about 400 terahertz (THz), and wavelengths are estimated to range between 1,000 micrometers (µm) and 760 nanometers (2.9921 inches),

Please notice that although the IR band is very large, the 5G frequency is included in that IR range.

Figure MG-7. Response to NIR light stimulation of functionalized AuNRs on HEK-293T cells. Comparison of the Fluo-4 AM signal after NIR treatment of NR720 functionalized with either anti-His6 or RGD peptide at 0.8, 1.5, and 4.0 W·cm-2. Anti-His6 antibody AuNRs produced fluorescence in HEK-293T cells above a threshold of ~ 1.0 W·cm-2 while RGD peptide AuNRs showed substantial fluorescence even at 0.8 W·cm-2.

The manipulation of magnetic nanoparticles (MNPs) using an external magnetic field, has been successfully demonstrated in various biomedical applications. Some have utilised this non-invasive external stimulus and there is an potential to build on this platform. The focus of this study is to understand the manipulation of MNPs by a time-varying static magnetic field and how, at different frequencies and displacement, this can alter cellular function. Here we explore, using numerical modeling, the physical mechanism which underlies this process, and we discuss potential improvements for its use in biomedical applications. From our data and other related studies, we infer that such phenomenon largely depends on the magnetic field gradient, magnetic susceptibility and size of the MNPs, magnet array oscillating frequency, viscosity of the medium surrounding MNPs, and distance between the magnetic field source and MNPs. Additionally, we demonstrate cytotoxicity in neuroblastoma (SH-SY5Y) and hepatocellular carcinoma (HepG2) cells in vitro induced by MNPs exposed to a magnetic field gradient and oscillating at various frequencies and displacement amplitudes. Even though this technique reliably produces MNP endocytosis and/or cytotoxicity, a better understanding is required to develop this system for precision manipulation of MNPs, ex vivo.

The manipulation of magnetic nanoparticles (MNPs) using external magnetic fields has led to the emergence of novel in vitro technologies. This technique which relies on non-invasive external stimuli, such as gradient magnetic field-assisted bioseparation [1] and gene transfection [2, 3]; alternating field gradient-mediated cytotoxicity [4]; dynamic magnetic field (DMF)-mediated cytotoxicity [5]; alternating magnetic field (AMF)-mediated cytotoxicity [6]; and controlled drug release [7]. The techniques mentioned above involve both permanent magnets and electromagnets with different working principles. Bioseparation consists of the use of a field gradient to capture specific biomolecules which are bound to MNPs [8]. Magnetofection involves the use of a magnetic field to attract magnetic nanoparticles and nucleic acid complexes towards cells to facilitate gene transfection [9, 10]. The alternating field gradient mediated cytotoxicity technique uses an alternating field gradient (Gz–95 G/cm) within a homogenous field (a 9.4 T preclinical MRI system) to align the nanoparticles parallel to the homogenous field and destroy cancer cells through an induced motion of magnetic nanoparticle aggregates within cells [4]. Dynamic magnetic fields (10–20 Hz, 30 mT) encourage torques, i.e., repeated incomplete rotational movements, of MNPs which enables remote stimulation of cell death by permeabilising the lysosome membrane and triggering apoptosis (programmed cell death) [5]. The application of alternating magnetic fields (50–1000 kHz) to MNPs which are bound to receptors on cell membranes or internalized within cells, has been used to activate chemical signaling in the cell, leading to apoptosis or the depositing of energy, triggering necrosis (cell death or tissue damage in an organ) [11].

The possible physical explanations for the static magnetic field gradient-mediated attracting/aligning of MNPs towards a magnet field have been discussed extensively [12,13,14,15]. Moreover, a possible mechanism for alternating field gradient mediated cytotoxicity has been examined [4]. However, an explanation for dynamic field-induced rotational motion has proven elusive; it is stated in [4] that a dynamic field generates a torque, equal to and that this enables the rotation of individual MNPs around their axis, but the dynamics of the magnetic fields used requires further exploration. Moreover, deposition of energy is possible when MNPs are exposed to alternating magnetic fields in the radio frequency range, since they dissipate heat due to susceptibility, hysteresis and friction losses [12].

This study focuses on the underlying conditions behind the manipulation of MNPs using unidirectional time-varying magnetic fields/field gradients and how this can induce magneto-mechanical cell death in cancer cells. The numerical model discussed here will direct researchers to this novel technique. Here, we used a time-varying (1–4 Hz) alternate pole magnet array plate populated with Nd–Fe–B permanent magnets (~ 450 mT) to create field gradients which result in enhanced attraction of magnetic nanoparticle towards cells which led to cytotoxicity in neuroblastoma (SH-SY5Y) and hepatocellular carcinoma (Hep G2) cancer cells.

Now, what does this mean to you with massive data collection?

Targeting individuals genetically and identifying their goals and behavior and deprogramming their genes to deactivate them and rob them of their free will.

For instance, if you believe you need to be good looking and a body builder in order to make a good first impression to achieve your goals, this technology allows a government agency to identify your genes and modify them so that your behavior is undone.

Now, the brain has the ability to regulate its own thoughts and behavior through effort. This, through computer calculation, can actively undo any effort your body makes to re-implement needed behavior.

This form of manipulation is barely detectable but may come in the sensation of weird vibrations in the nervous system and ear ringing.

It is far more evil than direct remote control because it doesn't require continuous stimulation of the brain. It can be coupled with it and used for absolute evil.

Its IS being used for absolute evil. We need to get a hole of some of these vaccines and check it for materials.

This technology is infact how the corona virus could be produced using 5G technology.

https://pubmed.ncbi.nlm.nih.gov/32668870/

RETRACTED: 5G Technology and induction of coronavirus in skin cells

If anyone has the original study please post it.

In this research, we show that 5G millimeter waves could be absorbed by dermatologic cells acting

like antennas, transferred to other cells and play the main role in producing Coronaviruses in biological

cells. DNA is built from charged electrons and atoms and has an inductor-like structure. This structure

could be divided into linear, toroid and round inductors. Inductors interact with external electromagnetic

waves, move and produce some extra waves within the cells. The shapes of these waves are similar to

shapes of hexagonal and pentagonal bases of their DNA source. These waves produce some holes in

liquids within the nucleus. To fill these holes, some extra hexagonal and pentagonal bases are produced.

These bases could join to each other and form virus-like structures such as Coronavirus. To produce

these viruses within a cell, it is necessary that the wavelength of external waves be shorter than the size

of the cell. Thus 5G millimeter waves could be good candidates for applying in constructing virus-like

structures such as Coronaviruses (COVID-19) within cells

>>59525

https://scienceintegritydigest.files.wordpress.com/2020/07/fioranelli.pdf

>>59522

Your Thoughts Activate Your Genes. You are speaking to your genes with every thought you have. The fast-growing field of epigenetics is proving that who you are is the product of the things that happen to you in your life, which change the way your genes operate. Genes are actually switched on or off depending on your life experiences, and your genes and lifestyle form a feedback loop.

https://thebestbrainpossible.com/how-your-thoughts-change-your-brain-cells-and-genes/

So, in effect, the second you try to change your behavior itll get reverted back. You'll be continuously frustrated wondering why your body never responds to your demands.

Are you a cis white male that spent 6 years body building? Is this a great achievement for you?

It wont be for long. Say goodbye to your ability to control working out and your ability to control your diet.

Obesity is a highly heritable disease driven by complex interactions between genetic and environmental factors. Human genome-wide association studies (GWAS) have identified a number of loci contributing to obesity; however, a major limitation of these studies is the inability to assess environmental interactions common to obesity. Using a systems genetics approach, we measured obesity traits, global gene expression, and gut microbiota composition in response to a high-fat/high-sucrose (HF/HS) diet of more than 100 inbred strains of mice. Here we show that HF/HS feeding promotes robust, strain-specific changes in obesity that are not accounted for by food intake and provide evidence for a genetically determined set point for obesity. GWAS analysis identified 11 genome-wide significant loci associated with obesity traits, several of which overlap with loci identified in human studies. We also show strong relationships between genotype and gut microbiota plasticity during HF/HS feeding and identify gut microbial phylotypes associated with obesity.

https://www.sciencedirect.com/science/article/pii/S1550413112004986

>>59527

It's quite different from 4G in that it's directional so that it can amply transmission toward known devices as necessary when signals falter. With 4G, the best that can be done is the device ramps up transmission power when it can't get a response from any local tower.

What does Pharma mean in Latin? pharmaceutical (adj.) “pertaining to pharmacy or the art of preparing drugs,” 1640s (pharmaceutic in the same sense is from 1540s), from Late Latin pharmaceuticus “of drugs,” from Greek pharmakeutikos, from pharmakeus “preparer of drugs, poisoner” (see pharmacy).