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File: 0a1d6a6af18b1ca⋯.jpg (7.53 KB, 255x143, 255:143, fight_fight_fight.jpg)

62d7e8  No.16324424[View All]

Welcome to Q Research General

We are researchers who deal in open-source information, reasoned argument, and dank memes. We do battle in the sphere of ideas and ideas only. We neither need nor condone the use of force in our work here.

"We hold these truths to be self-evident: that all men are created equal; that they are endowed by their Creator with certain unalienable rights; that among these are life, liberty, and the pursuit of happiness."

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701 posts and 384 image replies omitted. Click reply to view. ____________________________
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bf9c9c  No.16325191

>>16325182

Also look up Shannon Breem's interview with Cruz prior to the second impeachment vote…

Quite enlightening…

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7d17ae  No.16325192

File: 5892d896fef5fce⋯.png (37.6 KB, 951x203, 951:203, ClipboardImage.png)

Ron Paul & Rand Paul…Not on the list

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96d186  No.16325193

>>16325124

>you are deceived

no, i'm not. you are brainwashed, and triggered by words without knowing their true meaning.

you confuse "communism" with "communism." one is a word applied to demonize any enemy irrespective of actual ideology.

the other is a social system that is in fact consistent with the teaching of Jesus. you should visit an "evil" amish community.

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62d7e8  No.16325194

File: 9d7d5568d453d2c⋯.png (72.13 KB, 289x353, 289:353, ClipboardImage.png)

[Profile picture from source site (Twitter/Gettr/Truth Social)]Donald J. Trump/ @realDonaldTrump 05/22/2022 14:18:26

ID: Not Available

Truth Social: 108346966451100254

% buffered

00:00

00:00

00:00

Download

Image Name: 375bde2fedb261a1.mp4

Filename: 375bde2fedb261a1.mp4

(Cap 0:57)

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74f01c  No.16325195

File: 91ea18d9fb937eb⋯.png (1.91 MB, 928x924, 232:231, 91ea18d9fb937eb4239512c486….png)

here comes the baby formula!

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e829c9  No.16325196

File: 191105d1a1189f4⋯.jpg (322.15 KB, 1467x793, 1467:793, robinson3.jpg)

File: 4b0c8d938c93a0d⋯.jpg (49.51 KB, 567x567, 1:1, robinson5.jpg)

>>16325190

And these guys

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62d7e8  No.16325197

NOTABLES ARE NOT ENDORSEMENTS

>>16324437

#20651

>>16325073, >>16325078 Donald J. Trump: I think Twitter is going down. Bots, Spam, Fake Accounts (and more!), all add up to big trouble

>>16325147 "Elon Musk, from 50% to 90% of Twitter accounts for daily posts may in fact be Bots" found on glp (7:11)

>>16324472 GOP Rubber Stamps Joe Biden’s $900M for More Immigration from Ukraine

>>16324488 Rubio Accuses Google Of Election Bias, Censoring Campaign Emails

>>16324501, >>16324821, >>16325156 Monkeypox False Flag

>>16324707, >>16324733, >>16324750 Monkeypox Was a Table-Top Simulation Only Last Year

>>16324543 2003 Bioterror Researchers Build A More Lethal Mousepox

>>16324999 Google is now "Fact Checking" Monkeypox, anons have something

>>16325031, >>16325036 2021 Munich Security Conference Monkeypox ‘Pandemic Simulation’ Resurfaces as Real-Life Cases Rise

>>16324951 The clock and graphic are essential

>>16324520, >>16324540, >>16324635, >>16324677, >>16324828, >>16324844, >>16324909 Clockfag teaching the non autists

>>16324605 Peter Morales California teacher arrested for molesting four students in classroom

>>16324614 PF Reports Up from Okinawa and headed into South Korea. BEECH 200 Super King Air 170111. Call sign MAGIC31

>>16324650, >>16324670, >>16324699, >>16324795 Biden in Korea, Tokyo next

>>16324681, >>16324687, >>16324947 Examples of “Everything Woke Turns to Shit” ESPN and Youtube

>>16324583, >>16324588, >>16324621, >>16324831, >>16324906 Missy Owen / Hunter Biden emails comms "went out and bought a new car" = 187?

>>16324814 Sanhedrin Jews oppose WHO authority citing individual sovereignty over health care

>>16324757, >>16324829 Ted Cruz, banned from Russia

>>16324716 All the Republican senators justifying voting for $40 Billion to Ukraine

>>16324592, >>16325109, >>16324825, >>16324741, >>16324849, >>16325087, >>16325014, >>16324858, >>16324862, >>16324864, >>16324866, >>16324871, >>16324873 +++ and US citizens under personal sanctions, including a ban on entry into the Russian Federation

>>16324954 The Spook Lists!

>>16325020, >>16325039, >>16325042, >>16325050 Names not in the Russian list of sanctioned individuals

>>16324786 WANTED: Victims of Twitter censorship

>>16324807, >>16324809, >>16324927 Richard Seth Adler, Psychiatrist

>>16324838 Biden has Broken America

>>16324931, >>16325024 Kellyanne Conway takes aim at Jared Kushner in new book

>>16324939 Television presenter Erin Jayne Plummer, died 'suddenly' over the weekend

>>16324982 HillaryClinton worked with the @FBI to push a fake Russian collusion accusation against President Trump - and @maggieNYT got a Pulitzer for the fake story.

>>16325088 UN expert predicts global food crisis may be 10 weeks away

Baking

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5c0c43  No.16325198

File: b54a55915d38aca⋯.png (14.93 KB, 255x185, 51:37, ClipboardImage.png)

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081c52  No.16325199

File: c4c70938560882f⋯.jpeg (1.16 MB, 896x6498, 448:3249, Web_capture_22_5_2022_232….jpeg)

File: ab5d1b1c9cae41e⋯.jpeg (635.57 KB, 896x4325, 896:4325, Web_capture_22_5_2022_231….jpeg)

>>16325176

>>16325181

https://www.jove.com/t/59626/in-vitro-transcribed-rna-based-luciferase-reporter-assay-to-study

Immunology and Infection

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

We present a protocol to study mRNA translation regulation in poxvirus-infected cells using in vitro Transcribed RNA-based luciferase reporter assay. The assay can be used for studying translation regulation by cis-elements of an mRNA, including 5’-untranslated region (UTR) and 3’-UTR. Different translation initiation modes can also be examined using this method.

Abstract

Every poxvirus mRNA transcribed after viral DNA replication has an evolutionarily conserved, non-templated 5'-poly(A) leader in the 5'-UTR. To dissect the role of 5'-poly(A) leader in mRNA translation during poxvirus infection we developed an in vitro transcribed RNA-based luciferase reporter assay. This reporter assay comprises of four core steps: (1) PCR to amplify the DNA template for in vitro transcription; (2) in vitro transcription to generate mRNA using T7 RNA polymerase; (3) Transfection to introduce in vitro transcribed mRNA into cells; (4) Detection of luciferase activity as the indicator of translation. The RNA-based luciferase reporter assay described here circumvents issues of plasmid replication in poxvirus-infected cells and cryptic transcription from the plasmid. This protocol can be used to determine translation regulation by cis-elements in an mRNA including 5'-UTR and 3'-UTR in systems other than poxvirus-infected cells. Moreover, different modes of translation initiation like cap-dependent, cap-independent, re-initiation, and internal initiation can be investigated using this method.

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bf9c9c  No.16325200

>>16325193

Word Salad alert…

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664358  No.16325201

>>16325154

Shit bro. Gotta check it. Do they have a welcome list to go with the not welcome list?

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f24e98  No.16325202

File: 2f1f165ff5613a0⋯.png (150.89 KB, 640x480, 4:3, 008.png)

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548098  No.16325204

File: 3b038b70b71d875⋯.jpg (79.94 KB, 640x480, 4:3, gretch_page.jpg)

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513115  No.16325205

YouTube embed. Click thumbnail to play.

>>16324502

Lyrics:

Dawn of light lying between a silence and sold sources

Chased amid fusions of wonder

In moments hardly seen forgotten

Coloured in pastures of chance dancing leaves cast spells of challenge

Amused but real in thought, we fled from the sea whole

Dawn of thought transferred through moments of days undersearching earth

Revealing corridors of time provoking memories

Disjointed but with purpose

Craving penetrations offer links with the self instructors sharp and tender love

As we took to the air a picture of distance

Dawn of our power we amuse redescending as fast as misused expression

As only to teach love as to reveal passion chasing late into corners

And we danced from the ocean

Dawn of love sent within us colours of awakening among the many wont to follow

Only tunes of a different age

As the links span our endless caresses for the freedom of life everlasting

Talk to the sunlight caller

Soft summer mover distance mine

Called out a tune but I never saw the face

Heard but not replaced

I ventured to talk but I never lost my place

Cast out a spell rendered for the light of day

Lost in lights array

I ventured to see as the sound began to play

What happened to this song we once knew so well

Signed promise for moments caught within the spell

I must have waited all my life for this

Moment moment

The future poised with the splendour just begun

The light we were as one

And crowded through the curtains of liquid into sun

And for a moment when our world had filled the skies

Magic turned our eyes

To feast on the treasure set for our strange device

What happened to wonders we once knew so well

Did we forget what happened, surely we can tell

We must have waited all our lives for this

Moment moment moment

Starlight movement

Reasons

Release forward

Tallest rainbow

Sun shower seasons

Life flower reasons

They move fast, they tell me

But I just can't believe that I can feel it

There's someone to tell you

Amid the challenge we look around in unison with you

Getting over overhanging trees, let them rape the forest

Thoughts would send our fusion clearly to be home

Getting over wars we do not mean, or so it seems so clearly

Sheltered with our passion clearly to be home

They move fast, they tell me

But I just can't believe they really mean to

There's someone to tell you

And I just can't believe our song will leave you

Skyline teacher

Warland seeker

Send out poison

Cast iron leader

And through the rhythm of moving slowly

Sent through the rhythm work out the story

Move over glory to sons of old fighters past

Young Christians see it from the beginning

Old people feel it, that's what they're saying

Move over glory to sons of old fighters past

They move fast, they tell me

But I just can't believe they really mean to

There's someone to tell you

A course towards a universal season

Getting over overhanging trees, let them rape the forest

They might stand and leave them clearly to be home

Getting over wars they do not mean, we charm the movement suffers

Call out all our memories clearly to be home

We've moved fast, we need love

A part we offer is our only freedom

What happened to this song we once knew so well

Signed promise for moments caught within the spell

We must have waited all our lives for this

Moment moment

Past present movers moments we'll process the future,

But only through Him we know

Send flowered rainbows

A piece apart chased flowers of the dark and lights of songs

To follow and show all we feel for and know of

Cast round

You seekers of the truth accepting that reason will relive

And breathe and hope and chase and love

For you and you and you

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96d186  No.16325206

>>16325200

triggered tard alert

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5cfd92  No.16325207

File: 098c361deb8102c⋯.jpg (75.23 KB, 780x676, 15:13, Vladimir1.jpg)

>>16325152

so true, so true.

ty Vlad, some names we might have missed..

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af26f9  No.16325210

>>16325193

you should try your mossad shillspeak on somebody who buys it

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684814  No.16325211

>>16325202

Keking kekarious!!!!

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3afa23  No.16325212

File: 42dc03e268328f1⋯.jpg (127.98 KB, 1050x700, 3:2, Suicidejoebiden.jpg)

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bf9c9c  No.16325215

>>16325206

Must be part of the faggots that monitor this board, put a block in place and removed my notables.

No worries

You cannot block me forever…

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081c52  No.16325216

>>16325199

>>16325176

>>16325181

https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006602

The 5'-poly(A) leader of poxvirus mRNA confers a translational advantage that can be achieved in cells with impaired cap-dependent translation

Pragyesh Dhungel,Shuai Cao,Zhilong Yang

Version 2

Published: August 30, 2017

Abstract

The poly(A) leader at the 5’-untranslated region (5’-UTR) is an unusually striking feature of all poxvirus mRNAs transcribed after viral DNA replication (post-replicative mRNAs). These poly(A) leaders are non-templated and of heterogeneous lengths; and their function during poxvirus infection remains a long-standing question. Here, we discovered that a 5’-poly(A) leader conferred a selective translational advantage to mRNA in poxvirus-infected cells. A constitutive and uninterrupted 5’-poly(A) leader with 12 residues was optimal. Because the most frequent lengths of the 5’-poly(A) leaders are 8–12 residues, the result suggests that the poly(A) leader has been evolutionarily optimized to boost poxvirus protein production. A 5’-poly(A) leader also could increase protein production in the bacteriophage T7 promoter-based expression system of vaccinia virus, the prototypic member of poxviruses. Interestingly, although vaccinia virus post-replicative mRNAs do have 5’- methylated guanosine caps and can use cap-dependent translation, in vaccinia virus-infected cells, mRNA with a 5’-poly(A) leader could also be efficiently translated in cells with impaired cap-dependent translation. However, the translation was not mediated through an internal ribosome entry site (IRES). These results point to a fundamental mechanism poxvirus uses to efficiently translate its post-replicative mRNAs.

Could the 5’-poly(A) leader mediate cap-independent translation during poxvirus infection?

Many RNA viruses, such as piconaviruses, crucifer-infecting tobamovirus, hepatitis C virus, and Foot-and-mouth disease virus can synthesize their proteins through a cap-independent translation mode [41, 43–45]. The most studied mechanism is through viral IRESs, which usually bear highly complex structures to recruit 40S ribosome [46]. Some RNA viruses use other cap-independent mechanism such as 3’ cap-independent translational enhancer (3’CITE) in their mRNAs to recruit ribosome subunits [47, 48]. Cap-independent translation is less appreciated in DNA viruses. In this study, we show that a poly(A)-headed mRNA is efficiently translated in cells with impaired cap-dependent translation (Fig 8) as well as without an m7G cap (Fig 7), which strongly suggests that a short, unstructured 5’-poly(A) leader may mediate cap-independent translation in VACV-infected cells. In literature, Mulder et al. showed that VACV protein synthesis only requires a low level of intact translation initiation factor eIF4F [49]. In another in vitro study, Shirokikh et al. showed that the translation initiation complex could be formed on a 5’-poly(A) leader mRNA without the need of eIF4E, a rate-limiting and cap binding translation factor [50]. Additionally, in yeast, crucifer-infecting tobamovirus, and avian herpesvirus, an A-rich tract in some 5’-UTRs is suggested to function as an IRES [26, 41, 42], an RNA element allowing for a form of 5’ cap-independent translation initiation. Recruitment of poly(A) binding protein (PABP) through the A-rich tracts plays an important role in IRES-mediated cap-independent translation initiation. During the review process of this manuscript, Jha et al. reported that a small ribosomal subunit protein, receptor for activated C kinase (RACK1), is important for efficient translation of VACV post-replicative mRNAs [51]. RACK1 was previously found to be important for IRES-mediated cap-independent translation of several RNA viruses [52]. These findings support the possibility that the poly(A) can mediate cap-independent translation although it does not serve as an IRES (Fig 9).

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f0fd32  No.16325217

>>16325210

>>16325210

Kek

He has a point derp

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0c0c9c  No.16325220

>>16325216

They look so far into a microscope that they no longer see humanity.

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cb48c2  No.16325221

File: f3e2b71f171d7a2⋯.jpg (11.04 KB, 197x255, 197:255, download_jpeg_76.jpg)

>>16325124

Also newsweek this week

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c25b3e  No.16325222

YouTube embed. Click thumbnail to play.

>>16324424

It Will Come In Time==

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62d7e8  No.16325223

File: 3d47e3a1078dc40⋯.png (344.6 KB, 680x680, 1:1, ClipboardImage.png)

Fresh Bread

>>16325203

Q Research General #20652: I think Twitter is going down, all adds up to big trouble - PDJT Edition'

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873a10  No.16325224

File: 41801400b56a203⋯.jpeg (9.37 KB, 191x255, 191:255, giraff.jpeg)

four empire state buildings on its way to kills us all later this week…what about the giraffes?

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5c0c43  No.16325225

File: eda5326716bc4d8⋯.png (169.43 KB, 382x394, 191:197, ClipboardImage.png)

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f0fd32  No.16325227

>>16325224

Nothing can stop it I guess.

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081c52  No.16325228

File: 44683ffa9b2614f⋯.jpg (597.6 KB, 1748x800, 437:200, iamlegend_alternate_smith_….jpg)

https://www.yahoo.com/entertainment/first-human-patient-injected-revolutionary-140900099.html

First human patient injected with revolutionary cancer-killing virus

Scientists have injected the first human patient with a new cancer-killing virus. The virus, known as Vaxinia, has seen successful tests in animals. However, the true test of its efficacy begins with this new clinical trial.

It’s easy to hear the word virus and instantly think of something bad. After all, there are a lot of deadly viruses out there. However, scientists are using a new cancer-killing virus known as Vaxinia in an experimental cancer treatment.

The hope here is that the virus will amplify the body’s immune response against cancer. The virus itself has been engineered specifically to kill cancer cells. And, in previous animal trials, scientists have seen very promising results. These kinds of viruses have been a “smoking gun” in the fight against cancer for over a century.

However, the success of these viruses has been very limited, to say the least. This time around, though, the scientists have engineered the cancer-killing virus to not only harm cancer cells, but also to make them more recognizable to the body’s immune system. Researchers hope that this will help make the body’s response stronger, allowing it to fight back better.

Previous clinical trials of cancer drugs have shown promising results, too. But, Vaxinia could help open new doors, too.

Of course, before moving on to the human tests, the scientists tested the virus on animals. In many cases, they saw huge success at shrinking tumors in early animal and lab experiments. The cancer-killing virus has shown that it can reduce the size of lung, breast, ovarian, pancreatic, and colon cancer tumors.

With that success, the scientists decided to move on to human testing. Results seen in animals do not always directly translate to human patients. There are a lot of reasons for this, obviously, but the researchers are hopeful this virus could improve patients’ chance to fight back against cancer.

Currently, Vaxinia will be tested in a Phase 1 trial of just 100 cancer patients. These patients have metastatic or advanced solid tumors, and each has tried at least two other treatments. The researchers plan to administer the drug in two different groups. The first will receive just Vaxinia. The second group will get the cancer-killing virus plus an immunotherapy drug.

Of course, Phase 1 trials are mostly about safety and finding the optimal dose. As such, it might not prove the efficacy of the virus as a whole. However, it is an important next step in possibly finding an alternative method to fighting cancer. The trial is currently expected to complete by early 2025. So, it’ll be a while before we see any final results.

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62d7e8  No.16325229

File: 388d1c837dd6167⋯.png (910.23 KB, 1235x832, 95:64, ClipboardImage.png)

Fresh Bread

>>16325203

Q Research General #20652: I think Twitter is going down, all adds up to big trouble - PDJT Edition

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14bfa7  No.16325230

russia had already put sanctions on 398 members of congress on april 13th

they have said the list would be expanded

are these new sanctions tougher or just an expanded list?

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cb48c2  No.16325231

File: e85b109767bd56d⋯.jpg (51.99 KB, 524x476, 131:119, 6gtgo9.jpg)

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873a10  No.16325233

File: 5df6ebe4dc05032⋯.png (1.07 MB, 1080x762, 180:127, dfb0a1637406d18c5b10abe022….png)

>>16325186

>Mark Milley

a little white rage for 'the general'

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bf9c9c  No.16325235

>>16325227

Vomit has a way of finding the cracks in the best laid plans

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5cfd92  No.16325236

File: a96a509eb53b317⋯.png (5.68 KB, 1168x121, 1168:121, 955_Ron_Estes_Russia_sanct….PNG)

File: 6864be8271c097e⋯.png (1019.56 KB, 800x1000, 4:5, ClipboardImage.png)

File: ba6d43c7cb6e926⋯.png (194.79 KB, 1920x1152, 5:3, ClipboardImage.png)

File: b7e5b22b0db544f⋯.png (1.6 MB, 1280x720, 16:9, ClipboardImage.png)

File: 205fe433a504f44⋯.png (186.64 KB, 474x306, 79:51, ClipboardImage.png)

Ronald Gene Estes is an American politician, engineer, and farmer who has been the U.S. representative for Kansas's 4th congressional district since April 2017. A member of the Republican Party, he served as Kansas State Treasurer from 2011 to 2017.

A fifth-generation Kansan, Estes studied engineering and business at Tennessee Technological University. He began his career as a consultant and executive in various manufacturing and service industries. Estes was elected treasurer of Sedgwick County in 2004 and reelected in 2008. He was elected Kansas State Treasurer in 2010 and reelected in 2014. After U.S. Representative Mike Pompeo resigned to become Director of the Central Intelligence Agency, Estes won the special election for the seat and was sworn in on April 25, 2017. He is the dean of Kansas's House delegation.

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7d17ae  No.16325237

File: 753cec500cc2d31⋯.png (17.03 KB, 951x107, 951:107, ClipboardImage.png)

940 Schumer

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0c0c9c  No.16325238

>>16325230

This is a 'you cannot be here' list. Will be captured and ejected

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62d7e8  No.16325241

File: 7bd6cb01b1c52a3⋯.png (579.36 KB, 697x653, 697:653, ClipboardImage.png)

Fresh Bread

>>16325203

Q Research General #20652: I think Twitter is going down, all adds up to big trouble - PDJT Edition'

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7d17ae  No.16325242

File: fef168a0397a557⋯.png (26.28 KB, 961x224, 961:224, ClipboardImage.png)

note that the list doesn't follow standard english alphabetical order. ex: at 812 it goes from W to F

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089cd8  No.16325243

File: a14be754d0d1b2d⋯.png (721.14 KB, 914x1006, 457:503, 034C9766_E521_4ACE_BC39_B8….png)

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873a10  No.16325244

File: 691db42db01a55a⋯.jpeg (11.96 KB, 255x170, 3:2, 1b239176325f9d07eb90e4757….jpeg)

>>16325237

homophobes!

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ab6b5a  No.16325245

>>16324592

Putin certainly wouldn't be engaging in any joint disinfo ops by putting a few White Hats on it, right?

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873a10  No.16325246

File: 25eb732f0d455cd⋯.jpeg (12.93 KB, 255x175, 51:35, 84e0d710d89722fc2ab7bd4ce….jpeg)

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bf9c9c  No.16325249

>>16325238

Everyone on the list is commie

Russia eradicated the commies

They simply preventing those who will be expelled from the US from moving to their nation.

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62d7e8  No.16325252

File: b01e7e7a3053635⋯.png (1.33 MB, 1000x1000, 1:1, ClipboardImage.png)

Fresh Bread

>>16325203

Q Research General #20652: I think Twitter is going down, all adds up to big trouble - PDJT Edition

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c24a10  No.16325253

Maybe there are "Q supporters" on Putin's list because Q actually IS a DS psyop. Designed to get people to not take any tangible actions through informing the public of what lies ahead, so they don't fight back by telling everyone there's a plan in place to stop it.. Lying to us, making us feel all warm & cozy…then BAM. Something big that we can't come back from, happens…and we're all screwed.

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0c0c9c  No.16325255

>>16325249

Yes! They gotta go, but not here and not there

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081c52  No.16325257

01/21/2022 10:43 PM by an AC:

.

Everyone who has been injected with the (vaccine) has actually been injected with a virus of the pox family. The recipients are now know as vector’s. Through transfection the virus has been able to infect every single cell of the human body on such a nano scale that it’s undetectable until activated with a frequency, you guess what that may be but I think we all know what that will be. Studies regarding cDNA and transfecting vector’s from the mid 90’s. If you didn’t take the injection your clear. My guess? Start high infection with low mortality. Fowlpox or Monkeypox. Then change the frequency and high mortality with smallpox. These scumbags have made my life a living hell since I came across their documents and admissions. It’s only fair to warn the world. Wish you all the best of luck.

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873a10  No.16325258

File: 7de74a4cfd86d1a⋯.png (1 MB, 1200x1452, 100:121, ClipboardImage.png)

>>16325245

>White Hats

1950s idealism; no such thing

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