Let's see. I'm a pussy, give me the right music and sad people and that's enough. But at least I never cared for Bambi's mom…
First an old cartoon, did anyone else here see it? It friggin traumatized me, I watched it once as a little kid. It's named Samson & Sally and is about a little white whale whose idol is Moby Dick and yes, this happens in a child cartoon: Sally's parents get killed by hunters and the whole ocean is red with their blood, later Samson's mother is killed. Still, the hunter is a comedic character for some reason. Both almost choke during an oil spill. There's radiation poisoning. Moby Dick is senile and dies friggin smiling with open eyes while a disillusioned Samson tries to wake him up. Like, HOLY SHIT!
The episode "I remember you" from Adventure Time, where Marceline gets her Nam flashback and Ice King remembers nothing.
The final scene in Batman TAS's "Heart of Ice" for some reason. "I can only beg your forgiveness…"
Over the Garden Wall, the ending where all stories resolve while the frog sings over it.
Bojack Horseman, the whole fucking show. Hits too close to home, and when it doesn't, it triggers fear of my future.
Littlefoot's mom dies. I watched that movie twice and still can quote what she says. It burned into my mind.
Back then, I found the first An American Tail so sad, that I refused to watch it ever again. And I never did. But I love the second one.